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j.stemcr.2014.03.012.pdf | 3.84 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Otsuji, Tomomi G. | en |
dc.contributor.author | Bin, Jiang | en |
dc.contributor.author | Yoshimura, Azumi | en |
dc.contributor.author | Tomura, Misayo | en |
dc.contributor.author | Tateyama, Daiki | en |
dc.contributor.author | Minami, Itsunari | en |
dc.contributor.author | Yoshikawa, Yoshihiro | en |
dc.contributor.author | Aiba, Kazuhiro | en |
dc.contributor.author | Heuser, John E. | en |
dc.contributor.author | Nishino, Taito | en |
dc.contributor.author | Hasegawa, Kouichi | en |
dc.contributor.author | Nakatsuji, Norio | en |
dc.contributor.alternative | 中辻, 憲夫 | ja |
dc.date.accessioned | 2014-05-07T05:59:12Z | - |
dc.date.available | 2014-05-07T05:59:12Z | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/186982 | - |
dc.description | ヒト多能性幹細胞(ES/iPS細胞)の新たな三次元培養法 -大量培養・大規模生産を可能に-. 京都大学プレスリリース. 2014-04-25. | ja |
dc.description.abstract | Utilizing human pluripotent stem cells (hPSCs) in cell-based therapy and drug discovery requires large-scale cell production. However, scaling up conventional adherent cultures presents challenges of maintaining a uniform high quality at low cost. In this regard, suspension cultures are a viable alternative, because they are scalable and do not require adhesion surfaces. 3D culture systems such as bioreactors can be exploited for large-scale production. However, the limitations of current suspension culture methods include spontaneous fusion between cell aggregates and suboptimal passaging methods by dissociation and reaggregation. 3D culture systems that dynamically stir carrier beads or cell aggregates should be refined to reduce shearing forces that damage hPSCs. Here, we report a simple 3D sphere culture system that incorporates mechanical passaging and functional polymers. This setup resolves major problems associated with suspension culture methods and dynamic stirring systems and may be optimal for applications involving large-scale hPSC production. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2014 The Authors. | en |
dc.rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). | en |
dc.title | A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 2 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 734 | - |
dc.identifier.epage | 745 | - |
dc.relation.doi | 10.1016/j.stemcr.2014.03.012 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 28081436 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2014-04-25 | - |
dcterms.accessRights | open access | - |
dc.identifier.eissn | 2213-6711 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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