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Title: p54nrb/NonO and PSF promote U snRNA nuclear export by accelerating its export complex assembly.
Authors: Izumi, Hiroto
McCloskey, Asako
Shinmyozu, Kaori
Ohno, Mutsuhito  kyouindb  KAKEN_id
Author's alias: 大野, 睦人
Issue Date: Apr-2014
Publisher: Oxford University Press
Journal title: Nucleic acids research
Volume: 42
Issue: 6
Start page: 3998
End page: 4007
Abstract: The assembly of spliceosomal U snRNPs in metazoans requires nuclear export of U snRNA precursors. Four factors, nuclear cap-binding complex (CBC), phosphorylated adaptor for RNA export (PHAX), the export receptor CRM1 and RanGTP, gather at the m(7)G-cap-proximal region and form the U snRNA export complex. Here we show that the multifunctional RNA-binding proteins p54nrb/NonO and PSF are U snRNA export stimulatory factors. These proteins, likely as a heterodimer, accelerate the recruitment of PHAX, and subsequently CRM1 and Ran onto the RNA substrates in vitro, which mediates efficient U snRNA export in vivo. Our results reveal a new layer of regulation for U snRNA export and, hence, spliceosomal U snRNP biogenesis.
Rights: © The Author(s) 2014. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI(Published Version): 10.1093/nar/gkt1365
PubMed ID: 24413662
Appears in Collections:Journal Articles

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