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Title: p54nrb/NonO and PSF promote U snRNA nuclear export by accelerating its export complex assembly.
Authors: Izumi, Hiroto
McCloskey, Asako
Shinmyozu, Kaori
Ohno, Mutsuhito  kyouindb  KAKEN_id
Author's alias: 大野, 睦人
Issue Date: Apr-2014
Publisher: Oxford University Press
Journal title: Nucleic acids research
Volume: 42
Issue: 6
Start page: 3998
End page: 4007
Abstract: The assembly of spliceosomal U snRNPs in metazoans requires nuclear export of U snRNA precursors. Four factors, nuclear cap-binding complex (CBC), phosphorylated adaptor for RNA export (PHAX), the export receptor CRM1 and RanGTP, gather at the m(7)G-cap-proximal region and form the U snRNA export complex. Here we show that the multifunctional RNA-binding proteins p54nrb/NonO and PSF are U snRNA export stimulatory factors. These proteins, likely as a heterodimer, accelerate the recruitment of PHAX, and subsequently CRM1 and Ran onto the RNA substrates in vitro, which mediates efficient U snRNA export in vivo. Our results reveal a new layer of regulation for U snRNA export and, hence, spliceosomal U snRNP biogenesis.
Rights: © The Author(s) 2014. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/187373
DOI(Published Version): 10.1093/nar/gkt1365
PubMed ID: 24413662
Appears in Collections:Journal Articles

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