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dc.contributor.authorKinashi, Yukoen
dc.contributor.authorOkumura, Kakujien
dc.contributor.authorKubota, Yoshihisaen
dc.contributor.authorKitajima, Erikaen
dc.contributor.authorOkayasu, Ryuichien
dc.contributor.authorOno, Kojien
dc.contributor.authorTakahashi, Sentaroen
dc.contributor.alternative木梨, 友子ja
dc.date.accessioned2014-07-07T04:17:19Z-
dc.date.available2014-07-07T04:17:19Z-
dc.date.issued2014-06-
dc.identifier.issn0969-8043-
dc.identifier.urihttp://hdl.handle.net/2433/188913-
dc.description.abstract[Background]It is generally said that low LET radiation produce high dose-rate effect, on the other hand, no significant dose rate effect is observed in high LET radiation. Although high LET radiations are produced in BNCT, little is known about dose-rate effect of BNCT. [Materials and methods]T98G cells, which were tumor cells, were irradiated by neutron mixed beam with BPA. As normal tissue derived cells, Chinese hamster ovary (CHO-K1) cells and DNA double strand breaks (DNA-DSBs) repair deficient cells, xrs5 cells were irradiated by the neutrons (not including BPA). To DNA-DSBs analysis, T98G cells were stained immunochemically with 53BP1 antibody. The number of DNA-DSBs was determined by counting 53BP1 foci. [Results]There was no dose-rate effect in xrs5 cells. D0 difference between 4 cGy/min and 20 cGy/min irradiation were 0.5 and 5.9 at the neutron and gamma-ray irradiation for CHO-K1, and 0.3 at the neutron for T98G cells. D0 difference between 20 cGy/min and 80 cGy/min irradiation for T98G cells were 1.2 and 0.6 at neutron irradiation plus BPA and gamma-ray. The differences between neutron irradiations at the dose rate in T98G cells were supported by not only the cell viability but also 53BP1 foci assay at 24 h following irradiation to monitor DNA-DSBs. [Conclusion]Dose-rate effect of BNCT when T98G cells include 20 ppm BPA was greater than that of gamma-ray irradiation. Moreover, Dose-rate effect of the neutron beam when CHO-K1 cells did not include BPA was less than that of gamma-ray irradiation These present results may suggest the importance of dose-rate effect for more efficient BNCT and the side effect reduction.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier Ltd.en
dc.rights© 2013 Elsevier Ltd.en
dc.rightsThis is not the published version. Please cite only the published version.en
dc.rightsこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectDose-rate effecten
dc.subjectBNCTen
dc.subjectDNA-DSBsen
dc.subjectGliomaen
dc.titleDose-rate effect was observed in T98G glioma cells following BNCT.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA10874887-
dc.identifier.jtitleApplied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicineen
dc.identifier.volume88-
dc.identifier.spage81-
dc.identifier.epage85-
dc.relation.doi10.1016/j.apradiso.2013.11.117-
dc.textversionauthor-
dc.identifier.pmid24360864-
dcterms.accessRightsopen access-
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