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Title: Dose-rate effect was observed in T98G glioma cells following BNCT.
Authors: Kinashi, Yuko  KAKEN_id
Okumura, Kakuji
Kubota, Yoshihisa
Kitajima, Erika
Okayasu, Ryuichi
Ono, Koji
Takahashi, Sentaro  KAKEN_id
Author's alias: 木梨, 友子
Keywords: Dose-rate effect
BNCT
DNA-DSBs
Glioma
Issue Date: Jun-2014
Publisher: Elsevier Ltd.
Journal title: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
Volume: 88
Start page: 81
End page: 85
Abstract: [Background]It is generally said that low LET radiation produce high dose-rate effect, on the other hand, no significant dose rate effect is observed in high LET radiation. Although high LET radiations are produced in BNCT, little is known about dose-rate effect of BNCT. [Materials and methods]T98G cells, which were tumor cells, were irradiated by neutron mixed beam with BPA. As normal tissue derived cells, Chinese hamster ovary (CHO-K1) cells and DNA double strand breaks (DNA-DSBs) repair deficient cells, xrs5 cells were irradiated by the neutrons (not including BPA). To DNA-DSBs analysis, T98G cells were stained immunochemically with 53BP1 antibody. The number of DNA-DSBs was determined by counting 53BP1 foci. [Results]There was no dose-rate effect in xrs5 cells. D0 difference between 4 cGy/min and 20 cGy/min irradiation were 0.5 and 5.9 at the neutron and gamma-ray irradiation for CHO-K1, and 0.3 at the neutron for T98G cells. D0 difference between 20 cGy/min and 80 cGy/min irradiation for T98G cells were 1.2 and 0.6 at neutron irradiation plus BPA and gamma-ray. The differences between neutron irradiations at the dose rate in T98G cells were supported by not only the cell viability but also 53BP1 foci assay at 24 h following irradiation to monitor DNA-DSBs. [Conclusion]Dose-rate effect of BNCT when T98G cells include 20 ppm BPA was greater than that of gamma-ray irradiation. Moreover, Dose-rate effect of the neutron beam when CHO-K1 cells did not include BPA was less than that of gamma-ray irradiation These present results may suggest the importance of dose-rate effect for more efficient BNCT and the side effect reduction.
Rights: © 2013 Elsevier Ltd.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/188913
DOI(Published Version): 10.1016/j.apradiso.2013.11.117
PubMed ID: 24360864
Appears in Collections:Journal Articles

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