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Title: Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells.
Authors: Gotoh, Shimpei
Ito, Isao  kyouindb  KAKEN_id
Nagasaki, Tadao
Yamamoto, Yuki
Konishi, Satoshi
Korogi, Yohei
Matsumoto, Hisako  kyouindb  KAKEN_id
Muro, Shigeo  kyouindb  KAKEN_id
Hirai, Toyohiro  kyouindb  KAKEN_id
Funato, Michinori
Mae, Shin-Ichi  kyouindb  KAKEN_id
Toyoda, Taro  kyouindb  KAKEN_id  orcid (unconfirmed)
Sato-Otsubo, Aiko
Ogawa, Seishi  kyouindb  KAKEN_id
Osafune, Kenji  kyouindb  KAKEN_id
Mishima, Michiaki
Author's alias: 伊藤, 功朗
小川, 誠司
長船, 健二
三嶋, 理晃
Issue Date: 9-Sep-2014
Publisher: Elsevier Inc.
Journal title: Stem cell reports
Volume: 3
Issue: 3
Start page: 394
End page: 403
Abstract: No methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been reported. Based on a study of the stepwise induction of alveolar epithelial cells (AECs), we identified carboxypeptidase M (CPM) as a surface marker of NKX2-1(+) "ventralized" anterior foregut endoderm cells (VAFECs) in vitro and in fetal human and murine lungs. Using SFTPC-GFP reporter hPSCs and a 3D coculture system with fetal human lung fibroblasts, we showed that CPM(+) cells isolated from VAFECs differentiate into AECs, demonstrating that CPM is a marker of AEPCs. Moreover, 3D coculture differentiation of CPM(+) cells formed spheroids with lamellar-body-like structures and an increased expression of surfactant proteins compared with 2D differentiation. Methods to induce and isolate AEPCs using CPM and consequently generate alveolar epithelial spheroids would aid human pulmonary disease modeling and regenerative medicine.
Description: ヒトiPS細胞から肺胞上皮細胞を分化誘導し、単離する方法を確立 -肺の再生/創薬研究につながる大きな一歩-. 京都大学プレスリリース. 2014-08-22.
Rights: This is an open access article under the CC BY license (
DOI(Published Version): 10.1016/j.stemcr.2014.07.005
PubMed ID: 25241738
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