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Title: Evolutionary origin of higher-order repeat structure in alpha-satellite DNA of primate centromeres.
Authors: Koga, Akihiko  kyouindb  KAKEN_id
Hirai, Yuriko
Terada, Shoko
Jahan, Israt
Baicharoen, Sudarath
Arsaithamkul, Visit
Hirai, Hirohisa
Author's alias: 古賀, 章彦
Keywords: alpha-satellite DNA
higher-order repeat
centromere
hominoids
evolutionary origin
Issue Date: Aug-2014
Publisher: Oxford University Press
Journal title: DNA research : an international journal for rapid publication of reports on genes and genomes
Volume: 21
Issue: 4
Start page: 407
End page: 415
Abstract: Alpha-satellite DNA (AS) is a main DNA component of primate centromeres, consisting of tandemly repeated units of ~170 bp. The AS of humans contains sequences organized into higher-order repeat (HOR) structures, in which a block of multiple repeat units forms a larger repeat unit and the larger units are repeated tandemly. The presence of HOR in AS is widely thought to be unique to hominids (family Hominidae; humans and great apes). Recently, we have identified an HOR-containing AS in the siamang, which is a small ape species belonging to the genus Symphalangus in the family Hylobatidae. This result supports the view that HOR in AS is an attribute of hominoids (superfamily Hominoidea) rather than hominids. A single example is, however, not sufficient for discussion of the evolutionary origin of HOR-containing AS. In the present study, we developed an efficient method for detecting signs of large-scale HOR and demonstrated HOR of AS in all the three other genera. Thus, AS organized into HOR occurs widely in hominoids. Our results indicate that (i) HOR-containing AS was present in the last common ancestor of hominoids or (ii) HOR-containing AS emerged independently in most or all basal branches of hominoids. We have also confirmed HOR occurrence in centromeric AS in the Hylobatidae family, which remained unclear in our previous study because of the existence of AS in subtelomeric regions, in addition to centromeres, of siamang chromosomes.
Rights: © The Author 2014. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
URI: http://hdl.handle.net/2433/191075
DOI(Published Version): 10.1093/dnares/dsu005
PubMed ID: 24585002
Appears in Collections:Journal Articles

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