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j.ab.2014.08.029.pdf | 508.72 kB | Adobe PDF | 見る/開く |
タイトル: | Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing. |
著者: | Toda, Mitsuaki Arima, Yusuke Takiguchi, Hiromi Iwata, Hiroo |
著者名の別形: | 有馬, 祐介 岩田, 博夫 |
キーワード: | Surface plasmon field-enhanced fluorescence spectroscopy Convergent optical system Tumor marker Highly sensitive detection |
発行日: | 6-Sep-2014 |
出版者: | Elsevier Inc. |
誌名: | Analytical biochemistry |
巻: | 467 |
号: | 15 |
開始ページ: | 47 |
終了ページ: | 53 |
抄録: | Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) is a promising methodology for point-of-care (POC) testing. The SPFS devices that have been reported are equipped with an angle rotating stage to adjust the surface plasmon resonance (SPR) angle. In a clinical setting, however, the SPR angle determination is a tedious and time-consuming process. In this study, we employed an SPFS instrument with a convergent optical system that allows the omission of this procedure. We demonstrated that this instrumentation allowed the sensitive determination of low concentrations of α-fetoprotein in serum and reduced the variation effect caused by the protein concentrations in samples. The SPFS with a convergent optical system is suitable for POC testing. |
著作権等: | © 2014 Elsevier Inc. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/191329 |
DOI(出版社版): | 10.1016/j.ab.2014.08.029 |
PubMed ID: | 25197029 |
出現コレクション: | 学術雑誌掲載論文等 |
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