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dc.contributor.authorSakata, Tomokoja
dc.contributor.authorFujii, Kotaroja
dc.contributor.authorOhno, Mutsuhitoja
dc.contributor.authorKitabatake, Makotoja
dc.contributor.alternative北畠, 真ja
dc.description.abstractNonfunctional mutant ribosomal RNAs in 40S or 60S subunits are selectively degraded in eukaryotic cells (nonfunctional rRNA decay, NRD). We previously reported that NRD of 25S rRNA required cullin-E3 ligase Rtt101 and its associating factor Mms1, both of which are involved in DNA repair. Although Mms22, an accessory component of the E3 complex, was suggested to direct the E3 complex to DNA repair, the factor that directs the complex to 25S NRD currently remains unknown. We herein demonstrated that another accessory component, Crt10 was required for 25S NRD, but not for DNA repair, suggesting that this accessory component specifies the function of the E3 complex differently. We also identified two distinct Crt10-containing E3 complexes, one of which contained the Paf1 complex, a Pol-II binding complex that modulates the transcription of stress-related genes. Our results showed the convergence of multiple pathways for stresses that harm nucleic acids and provided a molecular framework for the substrate diversity of the E3 complex.ja
dc.publisherElsevier Inc.ja
dc.rights© 2014 Elsevier Inc. NOTICE: this is the author's version of a work that was accepted for publication in Biochemical and biophysical research communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and biophysical research communications, 457(1), 2015, doi:10.1016/j.bbrc.2014.12.072ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectQuality controlja
dc.subjectUbiquitin E3 ligaseja
dc.titleCrt10 directs the cullin-E3 ligase Rtt101 to nonfunctional 25S rRNA decay.ja
dc.type.niitypeJournal Articleja
dc.identifier.jtitleBiochemical and biophysical research communicationsja
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