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Title: Sequence-specific DNA alkylation by tandem Py-Im polyamide conjugates.
Authors: Taylor, Rhys Dylan
Kawamoto, Yusuke
Hashiya, Kaori
Bando, Toshikazu
Sugiyama, Hiroshi  kyouindb  KAKEN_id
Author's alias: 板東, 俊和
Keywords: alkylation
Issue Date: 18-Jun-2014
Publisher: wiley
Journal title: Chemistry -- an Asian journal
Volume: 9
Issue: 9
Start page: 2527
End page: 2533
Abstract: Tandem N-methylpyrrole-N-methylimidazole (Py-Im) polyamides with good sequence-specific DNA-alkylating activities have been designed and synthesized. Three alkylating tandem Py-Im polyamides with different linkers, which each contained the same moiety for the recognition of a 10 bp DNA sequence, were evaluated for their reactivity and selectivity by DNA alkylation, using high-resolution denaturing gel electrophoresis. All three conjugates displayed high reactivities for the target sequence. In particular, polyamide 1, which contained a β-alanine linker, displayed the most-selective sequence-specific alkylation towards the target 10 bp DNA sequence. The tandem Py-Im polyamide conjugates displayed greater sequence-specific DNA alkylation than conventional hairpin Py-Im polyamide conjugates (4 and 5). For further research, the design of tandem Py-Im polyamide conjugates could play an important role in targeting specific gene sequences.
Rights: This is the peer reviewed version of the following article: Taylor, R. D., Kawamoto, Y., Hashiya, K., Bando, T. and Sugiyama, H. (2014), Sequence-Specific DNA Alkylation by Tandem Py–Im Polyamide Conjugates. Chem. Asian J., 9: 2527–2533, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1002/asia.201402331
PubMed ID: 24942204
Appears in Collections:Journal Articles

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