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Title: K(+)-responsive off-to-on switching of hammerhead ribozyme through dual G-quadruplex formation requiring no heating and cooling treatment.
Authors: Yamaoki, Yudai  kyouindb  KAKEN_id  orcid (unconfirmed)
Nagata, Takashi  kyouindb  KAKEN_id  orcid (unconfirmed)
Mashima, Tsukasa  kyouindb  KAKEN_id
Katahira, Masato  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 片平, 正人
Keywords: G-quadruplex
Structural transition
RNA structure
Switching of activity
Issue Date: 4-Dec-2015
Publisher: Elsevier Inc.
Journal title: Biochemical and biophysical research communications
Volume: 468
Issue: 1-2
Start page: 27
End page: 31
Abstract: Functional RNAs that switch their activities in response to K(+) may sense the intracellular (100 mM) and extracellular (5 mM) K(+) concentrations and regulate their functions accordingly. Previously, we developed a quadruplex hammerhead ribozyme (QHR) whose conformational change, from a duplex to a G-quadruplex, triggered by K(+) results in expression of the activity. However, this QHR required heating and cooling treatment (annealing) to induce the K(+)-responsive conformational change and activity. Here, we developed a new quadruplex hammerhead ribozyme (QHR) system that does not require annealing to induce the K(+)-responsive conformational change and activity. This system is composed of QHR and a G-quadruplex-forming complementary DNA strand (QCS). In the absence of K(+), QCS formed a duplex with QHR, which suppressed the residual activity. Upon elevation of the K(+) concentration, QCS dissociated from QHR was trapped in a G-quadruplex, and then QHR could form a G-quadruplex and exerted the activity. The 11.6-fold higher activity was induced by K(+) with an EC50 value of 23 mM, but not by Na(+), which is desirable when the activity switching between the intra-/extracellular environment is aimed at. This is the first report of the activation of functional RNA through a 'dual G-quadruplex formation system'.
Rights: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
The full-text file will be made open to the public on 4 December 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.bbrc.2015.10.173
PubMed ID: 26546822
Appears in Collections:Journal Articles

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