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タイトル: K(+)-responsive off-to-on switching of hammerhead ribozyme through dual G-quadruplex formation requiring no heating and cooling treatment.
著者: Yamaoki, Yudai
Nagata, Takashi  kyouindb  KAKEN_id
Mashima, Tsukasa  kyouindb  KAKEN_id
Katahira, Masato  kyouindb  KAKEN_id
著者名の別形: 片平, 正人
キーワード: G-quadruplex
Structural transition
RNA structure
Switching of activity
Ribozyme
Potassium
発行日: 4-Dec-2015
出版者: Elsevier Inc.
誌名: Biochemical and biophysical research communications
巻: 468
号: 1-2
開始ページ: 27
終了ページ: 31
抄録: Functional RNAs that switch their activities in response to K(+) may sense the intracellular (100 mM) and extracellular (5 mM) K(+) concentrations and regulate their functions accordingly. Previously, we developed a quadruplex hammerhead ribozyme (QHR) whose conformational change, from a duplex to a G-quadruplex, triggered by K(+) results in expression of the activity. However, this QHR required heating and cooling treatment (annealing) to induce the K(+)-responsive conformational change and activity. Here, we developed a new quadruplex hammerhead ribozyme (QHR) system that does not require annealing to induce the K(+)-responsive conformational change and activity. This system is composed of QHR and a G-quadruplex-forming complementary DNA strand (QCS). In the absence of K(+), QCS formed a duplex with QHR, which suppressed the residual activity. Upon elevation of the K(+) concentration, QCS dissociated from QHR was trapped in a G-quadruplex, and then QHR could form a G-quadruplex and exerted the activity. The 11.6-fold higher activity was induced by K(+) with an EC50 value of 23 mM, but not by Na(+), which is desirable when the activity switching between the intra-/extracellular environment is aimed at. This is the first report of the activation of functional RNA through a 'dual G-quadruplex formation system'.
著作権等: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
The full-text file will be made open to the public on 4 December 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/207646
DOI(出版社版): 10.1016/j.bbrc.2015.10.173
PubMed ID: 26546822
出現コレクション:学術雑誌掲載論文等

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