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Title: Decreased expression of a gene caused by a T-DNA insertion in an adjacent gene in arabidopsis
Authors: Tamura, Kentaro  kyouindb  KAKEN_id
Kawabayashi, Takenori
Shikanai, Toshiharu  kyouindb  KAKEN_id
Hara-Nishimura, Ikuko
Author's alias: 西村, いくこ
Issue Date: 1-Feb-2016
Publisher: Public Library of Science
Journal title: PLOS ONE
Volume: 11
Issue: 2
Thesis number: e0147911
Abstract: ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a TDNA insertion in one gene can have effects on the expression of neighboring genes.
Rights: © 2016 Tamura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0147911
PubMed ID: 26828726
Appears in Collections:Journal Articles

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