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dc.contributor.author | Yoneda, Ryoma | en |
dc.contributor.author | Suzuki, Shiho | en |
dc.contributor.author | Mashima, Tsukasa | en |
dc.contributor.author | Kondo, Keiko | en |
dc.contributor.author | Nagata, Takashi | en |
dc.contributor.author | Katahira, Masato | en |
dc.contributor.author | Kurokawa, Riki | en |
dc.contributor.alternative | 真嶋, 司 | ja |
dc.contributor.alternative | 近藤, 敬子 | ja |
dc.contributor.alternative | 永田, 崇 | ja |
dc.contributor.alternative | 片平, 正人 | ja |
dc.date.accessioned | 2016-06-07T02:59:43Z | - |
dc.date.available | 2016-06-07T02:59:43Z | - |
dc.date.issued | 2016-01-25 | - |
dc.identifier.issn | 2045-3701 | - |
dc.identifier.uri | http://hdl.handle.net/2433/214478 | - |
dc.description.abstract | Background: Translocated in LipoSarcoma (TLS, also known as FUsed in Sarcoma) is an RNA/DNA binding protein whose mutation cause amyotrophic lateral sclerosis. In previous study, we demonstrated that TLS binds to long noncoding RNA, promoter-associated ncRNA-D (pncRNA-D), transcribed from the 5' upstream region of cyclin D1 (CCND1), and inhibits the expression of CCND1. Results: In order to elucidate the binding specificity between TLS and pncRNA-D, we divided pncRNA-D into seven fragments and examined the binding with full-length TLS, TLS-RGG2-zinc finger-RGG3, and TLS-RGG3 by RNA pull down assay. As a result, TLS was able to bind to all the seven fragments, but the fragments containing reported recognition motifs (GGUG and GGU) tend to bind more solidly. The full-length TLS and TLS-RGG2-zinc finger-RGG3 showed a similar interaction with pncRNA-D, but the binding specificity of TLS-RGG3 was lower compared to the full-length TLS and TLS-RGG2-zinc finger-RGG3. Mutation in GGUG and GGU motifs dramatically decreased the binding, and unexpectedly, we could only detect weak interaction with the RNA sequence with stem loop structure. Conclusion: The binding of TLS and pncRNA-D was affected by the presence of GGUG and GGU sequences, and the C terminal domains of TLS function in the interaction with pncRNA-D. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central Ltd. | en |
dc.rights | © 2016 Yoneda et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.subject | TLS/FUS | en |
dc.subject | Long noncoding RNA | en |
dc.subject | pncRNA | en |
dc.title | The binding specificity of Translocated in LipoSarcoma/FUsed in Sarcoma with lncRNA transcribed from the promoter region of cyclin D1 | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cell and Bioscience | en |
dc.identifier.volume | 6 | - |
dc.relation.doi | 10.1186/s13578-016-0068-8 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 4 | - |
dc.identifier.pmid | 26816614 | - |
dcterms.accessRights | open access | - |
dc.identifier.eissn | 2045-3701 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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