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Title: Female spontaneously diabetic Torii fatty rats develop nonalcoholic steatohepatitis-like hepatic lesions
Authors: Ishii, Yukihito
Motohashi, Yu
Muramatsu, Makoto
Katsuda, Yoshiaki
Miyajima, Katsuhiro
Sasase, Tomohiko
Yamada, Takahisa
Matsui, Tohru
Kume, Shinichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-8759-8476 (unconfirmed)
Ohta, Takeshi
Author's alias: 松井, 徹
久米, 新一
Keywords: Fatty liver
Fibrosis
Metabolic syndrome
Nonalcoholic steatohepatitis
Spontaneously diabetic torii fatty rat
Issue Date: 14-Aug-2015
Publisher: WJG Press
Journal title: World Journal of Gastroenterology
Volume: 21
Issue: 30
Start page: 9067
End page: 9078
Abstract: AIM: To investigate the histological features of the liver in spontaneously diabetic Torii (SDT) fatty rats compared with age-matched Sprague-Dawley (SD) rats. METHODS: Female SDT Lepr[fa](SDT fatty) rats and age-matched SD rats were fed ad libitum . Body weight and biochemical parameters, such as serum glucose, triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels as well as fatty acid and TG accumulation in the liver were evaluated at 8 wk of age in the non-fasting state and at 8-wk intervals from 8 to 40 wk of age. Histopathological examinations of the liver were performed using hematoxylin and eosin and Sirius Red staining as well as double staining for ED-1 and toluidine blue. The expression of genes involved in TG synthesis, inflammation, and fibrosis was examined in the liver. RESULTS: SDT fatty rats showed significantly increased body weight compared with SD rats. Serum glucose, TG, and TC levels were significantly higher in SDT fatty rats compared with SD rats. The serum AST and ALT levels in SDT fatty rats were significantly elevated at 8 wk of age compared with the levels in SD rats. Hepatic TG content was marked in SDT fatty rats from 8 to 32 wk of age. Histopathologically, severe hepatosteatosis accompanied by inflammation was observed at 8 wk of age, and fibrosis started to occur at 32 wk of age. Furthermore, Sirius Red and ED-1 staining were increased in the liver at 32 wk of age. Hepatic gene expression related to TG synthesis, inflammation and fibrosis tended to increase in SDT fatty rats compared with SD rats, and the gene expression related to TG secretion was decreased in SDT fatty rats compared with SD rats. CONCLUSION: Female SDT fatty rats have the potential to become an important animal model of nonalcoholic steatohepatitis with type 2 diabetes and obesity.
Rights: © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved. This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
URI: http://hdl.handle.net/2433/217350
DOI(Published Version): 10.3748/wjg.v21.i30.9067
PubMed ID: 26290633
Appears in Collections:Journal Articles

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