ダウンロード数: 153
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s12967-016-1043-1.pdf | 816.82 kB | Adobe PDF | 見る/開く |
タイトル: | A multiplex urinary immunoassay for bladder cancer detection: analysis of a Japanese cohort |
著者: | Goodison, Steve Ogawa, Osamu Matsui, Yoshiyuki Kobayashi, Takashi Miyake, Makito Ohnishi, Sayuri Fujimoto, Kiyohide Dai, Yunfeng Shimizu, Yoshiko Tsukikawa, Kazue Furuya, Hideki Rosser, Charles J. |
著者名の別形: | 小川, 修 松井, 喜之 小林, 恭 |
キーワード: | Biomarkers Bladder cancer Multiplex Protein Urine |
発行日: | 7-Oct-2016 |
出版者: | Springer Nature |
誌名: | Journal of Translational Medicine |
巻: | 14 |
論文番号: | 287 |
抄録: | Background;; Bladder cancer (BCa) is among the most commonly diagnosed malignancies worldwide, and due the high rate of post-operative disease recurrence, it is one of the most prevalent in many countries. The development of non-invasive molecular assays that can accurately detect and monitor BCa would be a major advance, benefiting both patients and healthcare systems. We have previously identified a urinary protein biomarker panel that is being developed for application in at-risk patient cohorts. Here, we investigated the potential utility of the multiplex assay in a Japanese cohort. Methods;; The Japanese study cohort collected from urology clinics at two institutions was comprised of a total of 288 subjects. The protein biomarker panel (IL8, MMP9, MMP10, ANG, APOE, SDC1, A1AT, PAI1, CA9, VEGFA) was monitored in voided urine samples collected prior to cystoscopy using a custom multiplex ELISA assay. The diagnostic performance of the biomarker panel was assessed using receiver operator curves, predictive modeling and descriptive statistics. Results;; Urinary biomarker concentrations were significantly elevated in cases versus controls, and in cases with high-grade and muscle-invasive tumors. The AUC for the 10-biomarker assay was 0.892 (95 % confidence interval 0.850–0.934), with an overall diagnostic sensitivity specificity of 0.85 and 0.81, respectively. A predictive model trained on the larger institutional cohort correctly identified 99 % of the cases from the second institution. Conclusions;; Urinary levels of a 10-biomarker panel enabled discrimination of patients with BCa. The multiplex urinary diagnostic assay has the potential to be developed for the non-invasive detection of BCa in at-risk Japanese patients. |
著作権等: | © 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
URI: | http://hdl.handle.net/2433/226288 |
DOI(出版社版): | 10.1186/s12967-016-1043-1 |
PubMed ID: | 27717367 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。