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Title: Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods
Authors: Nakatsuji, Hirotaka
Kawabata Galbraith, Kelly
Kurisu, Junko
Imahori, Hiroshi  kyouindb  KAKEN_id
Murakami, Tatsuya
Kengaku, Mineko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-1666-3648 (unconfirmed)
Author's alias: 中辻, 博貴
川端, ケリー
村上, 達也
見学, 美根子
Issue Date: 5-Jul-2017
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 7
Thesis number: 4694
Abstract: Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, followed by activation by illumination. In this study, we show that gold nanorods (AuNRs) functionalized with two conventional lipids, oleate and 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP), are capable of efficient transfection and quick photoactivation of the HSP promoter. Use of our AuNRs (DOTAP-AuNRs) was comparable to Lipofectamine 2000 in terms of transfection efficiency, while lower in cytotoxicity. Subsequent near-infrared laser (NIR) illumination of the cells transfected by DOTAP-AuNRs for 10 s induced time- and site-specific transgene expression without significant phototoxicity, to a degree similar to that of heating the entire culture dish for 30 min. Our mechanistic studies suggest that efficient transfection and quick photoactivation of the HSP promoter (HSP70b’) are due to the promoted endosomal escape of DOTAP-AuNRs. We propose a novel protocol for NIR-inducible, site-directed gene expression using an unprecedented complex of the three conventional components capable of both transfection and photothermal heating.
Description: 遺伝子を細胞内に運び、数秒の光照射で発現させる金ナノ粒子. 京都大学プレスリリース. 2017-07-10.
Rights: © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/226370
DOI(Published Version): 10.1038/s41598-017-04912-1
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2017/170705_1.html
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