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j.stemcr.2016.09.002.pdf4.49 MBAdobe PDF見る/開く
タイトル: Early Development of Definitive Erythroblasts from Human Pluripotent Stem Cells Defined by Expression of Glycophorin A/CD235a, CD34, and CD36
著者: Mao, Bin
Huang, Shu
Lu, Xulin
Sun, Wencui
Zhou, Ya
Pan, Xu
Yu, Jinfeng
Lai, Mowen
Chen, Bo
Zhou, Qiongxiu
Mao, Song
Bian, Guohui
Zhou, Jiaxi
Nakahata, Tatsutoshi
Ma, Feng
著者名の別形: 中畑, 龍俊
キーワード: hESC
hiPSC
hematopoiesis
development
erythroblasts
erythropoiesis
endothelial cells
GPA
CD36
CD34
発行日: 8-Nov-2016
出版者: Elsevier BV
誌名: Stem Cell Reports
巻: 7
号: 5
開始ページ: 869
終了ページ: 883
抄録: The development of human erythroid cells has been mostly examined in models of adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the development and maturation of erythroblasts derived from human pluripotent stem cells (hPSCs) by an efficient co-culture system. These hPSC-derived early erythroblasts initially showed definitive characteristics with a glycophorin A+ (GPA+) CD34lowCD36− phenotype and were distinct from adult CD34+ cell-derived ones. After losing CD34 expression, early GPA+CD36− erythroblasts matured into GPA+CD36low/+ stage as the latter expressed higher levels of β-globin along with a gradual loss of mesodermal and endothelial properties, and terminally suppressed CD36. We establish a unique in vitro model to trace the early development of hPSC-derived erythroblasts by serial expression of CD34, GPA, and CD36. Our findings may provide insight into the understanding of human early erythropoiesis and, ultimately, therapeutic potential.
著作権等: © 2016 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/226436
DOI(出版社版): 10.1016/j.stemcr.2016.09.002
出現コレクション:学術雑誌掲載論文等

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