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j.celrep.2017.02.052.pdf | 4.72 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Obayashi, Eiji | en |
dc.contributor.author | Luna, Rafael E. | en |
dc.contributor.author | Nagata, Takashi | en |
dc.contributor.author | Martin-Marcos, Pilar | en |
dc.contributor.author | Hiraishi, Hiroyuki | en |
dc.contributor.author | Singh, Chingakham Ranjit | en |
dc.contributor.author | Erzberger, Jan Peter | en |
dc.contributor.author | Zhang, Fan | en |
dc.contributor.author | Arthanari, Haribabu | en |
dc.contributor.author | Morris, Jacob | en |
dc.contributor.author | Pellarin, Riccardo | en |
dc.contributor.author | Moore, Chelsea | en |
dc.contributor.author | Harmon, Ian | en |
dc.contributor.author | Papadopoulos, Evangelos | en |
dc.contributor.author | Yoshida, Hisashi | en |
dc.contributor.author | Nasr, Mahmoud L. | en |
dc.contributor.author | Unzai, Satoru | en |
dc.contributor.author | Thompson, Brytteny | en |
dc.contributor.author | Aube, Eric | en |
dc.contributor.author | Hustak, Samantha | en |
dc.contributor.author | Stengel, Florian | en |
dc.contributor.author | Dagraca, Eddie | en |
dc.contributor.author | Ananbandam, Asokan | en |
dc.contributor.author | Gao, Philip | en |
dc.contributor.author | Urano, Takeshi | en |
dc.contributor.author | Hinnebusch, Alan G. | en |
dc.contributor.author | Wagner, Gerhard | en |
dc.contributor.author | Asano, Katsura | en |
dc.contributor.alternative | 永田, 崇 | ja |
dc.date.accessioned | 2017-10-30T07:09:51Z | - |
dc.date.available | 2017-10-30T07:09:51Z | - |
dc.date.issued | 2017-03-14 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | http://hdl.handle.net/2433/227736 | - |
dc.description.abstract | During eukaryotic translation initiation, eIF3 binds the solvent-accessible side of the 40S ribosome and recruits the gate-keeper protein eIF1 and eIF5 to the decoding center. This is largely mediated by the N-terminal domain (NTD) of eIF3c, which can be divided into three parts: 3c0, 3c1, and 3c2. The N-terminal part, 3c0, binds eIF5 strongly but only weakly to the ribosome-binding surface of eIF1, whereas 3c1 and 3c2 form a stoichiometric complex with eIF1. 3c1 contacts eIF1 through Arg-53 and Leu-96, while 3c2 faces 40S protein uS15/S13, to anchor eIF1 to the scanning pre-initiation complex (PIC). We propose that the 3c0:eIF1 interaction diminishes eIF1 binding to the 40S, whereas 3c0:eIF5 interaction stabilizes the scanning PIC by precluding this inhibitory interaction. Upon start codon recognition, interactions involving eIF5, and ultimately 3c0:eIF1 association, facilitate eIF1 release. Our results reveal intricate molecular interactions within the PIC, programmed for rapid scanning-arrest at the start codon. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2017 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | en |
dc.subject | translation initiation | en |
dc.subject | ribosome | en |
dc.subject | eIF5 | en |
dc.subject | eIF3 | en |
dc.subject | eIF1 | en |
dc.subject | start codon selection | en |
dc.subject | ribosomal pre-initiation complex | en |
dc.title | Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5 | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cell Reports | en |
dc.identifier.volume | 18 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 2651 | - |
dc.identifier.epage | 2663 | - |
dc.relation.doi | 10.1016/j.celrep.2017.02.052 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 28297669 | - |
dcterms.accessRights | open access | - |
dc.identifier.eissn | 2211-1247 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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