ダウンロード数: 85
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s41598-018-21142-1.pdf | 3.14 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Kondo, Keiko | en |
dc.contributor.author | Mashima, Tsukasa | en |
dc.contributor.author | Oyoshi, Takanori | en |
dc.contributor.author | Yagi, Ryota | en |
dc.contributor.author | Kurokawa, Riki | en |
dc.contributor.author | Kobayashi, Naohiro | en |
dc.contributor.author | Nagata, Takashi | en |
dc.contributor.author | Katahira, Masato | en |
dc.contributor.alternative | 近藤, 敬子 | ja |
dc.contributor.alternative | 真嶋, 司 | ja |
dc.contributor.alternative | 永田, 崇 | ja |
dc.contributor.alternative | 片平, 正人 | ja |
dc.date.accessioned | 2018-09-10T06:12:30Z | - |
dc.date.available | 2018-09-10T06:12:30Z | - |
dc.date.issued | 2018-02-12 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/234273 | - |
dc.description.abstract | The length of a telomere is regulated via elongation and shortening processes. Telomeric DNA and telomeric repeat-containing RNA (TERRA), which both contain G-rich repeated sequences, form G-quadruplex structures. Previously, translocated in liposarcoma (TLS) protein, also known as fused in sarcoma (FUS) protein, was found to form a ternary complex with the G-quadruplex structures of telomeric DNA and TERRA. We then showed that the third RGG motif of TLS, the RGG3 domain, is responsible for the complex formation. However, the structural basis for their binding remains obscure. Here, NMR-based binding assaying revealed the interactions in the binary and ternary complexes of RGG3 with telomeric DNA or/and TERRA. In the ternary complex, tyrosine bound exclusively to TERRA, while phenylalanine bound exclusively to telomeric DNA. Thus, tyrosine and phenylalanine each play a central role in the recognition of TERRA and telomeric DNA, respectively. Surprisingly in the binary complexes, RGG3 used both tyrosine and phenylalanine residues to bind to either TERRA or telomeric DNA. We propose that the plastic roles of tyrosine and phenylalanine are important for RGG3 to efficiently form the ternary complex, and thereby regulate the telomere shortening. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.title | Plastic roles of phenylalanine and tyrosine residues of an RGG motif of TLS/FUS in binary and ternary complex formation with the G-quadruplex structures of telomeric DNA and TERRA | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific reports | en |
dc.identifier.volume | 8 | - |
dc.relation.doi | 10.1038/s41598-018-21142-1 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 2864 | - |
dc.address | Institute of Advanced Energy, Kyoto University | en |
dc.address | Institute of Advanced Energy, Kyoto University・Graduate School of Energy Science, Kyoto University | en |
dc.address | Department of Chemistry, Graduate School of Science, Shizuoka University | en |
dc.address | Department of Chemistry, Graduate School of Science, Shizuoka University | en |
dc.address | Division of Gene Structure and Function, Research Center for Genomic Medicine, Saitama Medical University | en |
dc.address | Institute for Protein Research, Osaka University | en |
dc.address | Institute of Advanced Energy, Kyoto University・Graduate School of Energy Science, Kyoto University | en |
dc.address | Institute of Advanced Energy, Kyoto University・Graduate School of Energy Science, Kyoto University | en |
dc.identifier.pmid | 29434328 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 15H01256 | - |
datacite.awardNumber | 16H00833 | - |
datacite.awardNumber | 16K14678 | - |
datacite.awardNumber | 15H01634 | - |
datacite.awardNumber | 26440026 | - |
datacite.awardNumber | 15H00813 /16K07269 | - |
datacite.awardNumber | 15K18485 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。