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dc.contributor.author | Watanabe, Sho | en |
dc.contributor.author | Ohtani, Yuta | en |
dc.contributor.author | Aoki, Wataru | en |
dc.contributor.author | Uno, Yuko | en |
dc.contributor.author | Sukekiyo, Yasunori | en |
dc.contributor.author | Kubokawa, Seiichi | en |
dc.contributor.author | Ueda, Mitsuyoshi | en |
dc.contributor.alternative | 渡辺, 祥 | ja |
dc.contributor.alternative | 大谷, 優太 | ja |
dc.contributor.alternative | 青木, 航 | ja |
dc.contributor.alternative | 植田, 充美 | ja |
dc.date.accessioned | 2018-09-14T02:34:39Z | - |
dc.date.available | 2018-09-14T02:34:39Z | - |
dc.date.issued | 2018-09-07 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2433/234559 | - |
dc.description | 胎児脳発達や抗酸化機能を持つ野菜の開発に成功 --野菜工場へのマルチオミックス解析の展開--. 京都大学プレスリリース. 2018-09-13. | ja |
dc.description.abstract | Betacyanins have been reported as water-soluble, nitrogenous pigments found in the order Caryophyllales, and they are known for powerful natural antioxidant. The biofortification of secondary metabolites such as anthocyanins and betacyanins has recently been performed in food crops by metabolic engineering through genetic modification. However, the distribution of genetically modified foods is strictly regulated. Therefore, we aimed to develop a new method for biofortifying natural antioxidants, betacyanins, without genetic modification. We first detected the presence of betacyanins in red-tube spinach (Spinacia oleracea) through ultraviolet-visible spectroscopy and mass spectrometry. We then hydroponically cultivated plants in the presence of three candidate compounds for betacyanin biofortification: dopamine, Ca2+, and sucrose. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) and antioxidant activity analyses showed that sucrose was most successful in biofortifying betacyanins, and reverse transcription polymerase chain reaction (RT-PCR) indicated that several genes involved in betacyanin biosynthesis were induced by sucrose. Therefore, strategic hydroponics represents a new approach for cultivating betacyanin-enriched vegetables. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | © 2018 Watanabe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.title | Detection of betacyanin in red-tube spinach (Spinacia oleracea) and its biofortification by strategic hydroponics | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PLOS ONE | en |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 9 | - |
dc.relation.doi | 10.1371/journal.pone.0203656 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e0203656 | - |
dc.identifier.pmid | 30192863 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2018-09-13-0 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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