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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-018-28245-9.pdf | 1.52 MB | Adobe PDF | 見る/開く |
| タイトル: | Actin polymerization is activated by terahertz irradiation |
| 著者: | Yamazaki, Shota Harata, Masahiko Idehara, Toshitaka Konagaya, Keiji Yokoyama, Ginji Hoshina, Hiromichi Ogawa, Yuichi    https://orcid.org/0000-0002-2238-2615 (unconfirmed) |
| 著者名の別形: | 小長谷, 圭志 小川, 雄一 |
| 発行日: | 3-Jul-2018 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 8 |
| 論文番号: | 9990 |
| 抄録: | Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells. |
| 著作権等: | © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/234722 |
| DOI(出版社版): | 10.1038/s41598-018-28245-9 |
| PubMed ID: | 29968762 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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