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タイトル: | ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock |
著者: | Matsumiya, Marina Tomita, Takehito Yoshioka-Kobayashi, Kumiko Isomura, Akihiro ![]() Kageyama, Ryoichiro ![]() ![]() |
著者名の別形: | 松宮, 舞奈 吉岡-小林, 久美子 磯村, 彰宏 影山, 龍一郎 |
キーワード: | Chemical library screening Embryonic stem cell Induced presomitic mesoderm Segmentation clock Self-organization Synchronized oscillation |
発行日: | 14-Feb-2018 |
出版者: | The Company of Biologists |
誌名: | Development |
巻: | 145 |
号: | 4 |
論文番号: | dev156836 |
抄録: | Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock. |
著作権等: | © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
URI: | http://hdl.handle.net/2433/235334 |
DOI(出版社版): | 10.1242/dev.156836 |
PubMed ID: | 29437832 |
出現コレクション: | 学術雑誌掲載論文等 |
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