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Title: ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock
Authors: Matsumiya, Marina
Tomita, Takehito
Yoshioka-Kobayashi, Kumiko
Isomura, Akihiro  kyouindb  KAKEN_id
Kageyama, Ryoichiro  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 松宮, 舞奈
吉岡-小林, 久美子
磯村, 彰宏
影山, 龍一郎
Keywords: Chemical library screening
Embryonic stem cell
Induced presomitic mesoderm
Segmentation clock
Synchronized oscillation
Issue Date: 14-Feb-2018
Publisher: The Company of Biologists
Journal title: Development
Volume: 145
Issue: 4
Thesis number: dev156836
Abstract: Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Rights: © 2018. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
DOI(Published Version): 10.1242/dev.156836
PubMed ID: 29437832
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