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タイトル: | In Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens |
著者: | Loo, Yuet Ying Rukayadi, Yaya Nor-Khaizura, Mahmud-Ab-Rashid Kuan, Chee Hao Chieng, Buong Woei Nishibuchi, Mitsuaki Radu, Son |
著者名の別形: | 西渕, 光昭 |
キーワード: | silver nanoparticles tea leave extracts antimicrobial activity foodborne pathogens Gram-negative time-kill curves |
発行日: | 16-Jul-2018 |
出版者: | Frontiers Media SA |
誌名: | Frontiers in Microbiology |
巻: | 9 |
論文番号: | 1555 |
抄録: | Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection. |
著作権等: | © 2018 Loo, Rukayadi, Nor-Khaizura, Kuan, Chieng, Nishibuchi and Radu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
URI: | http://hdl.handle.net/2433/236376 |
DOI(出版社版): | 10.3389/fmicb.2018.01555 |
PubMed ID: | 30061871 |
出現コレクション: | 学術雑誌掲載論文等 |
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