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タイトル: In Vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens
著者: Loo, Yuet Ying
Rukayadi, Yaya
Nor-Khaizura, Mahmud-Ab-Rashid
Kuan, Chee Hao
Chieng, Buong Woei
Nishibuchi, Mitsuaki
Radu, Son
著者名の別形: 西渕, 光昭
キーワード: silver nanoparticles
tea leave extracts
antimicrobial activity
foodborne pathogens
Gram-negative
time-kill curves
発行日: 16-Jul-2018
出版者: Frontiers Media SA
誌名: Frontiers in Microbiology
巻: 9
論文番号: 1555
抄録: Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against Escherichia coli, Klebsiella pneumoniae, Salmonella Typhimurium, and Salmonella Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL⁻¹ was >3 Log₁₀ units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.
著作権等: © 2018 Loo, Rukayadi, Nor-Khaizura, Kuan, Chieng, Nishibuchi and Radu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
URI: http://hdl.handle.net/2433/236376
DOI(出版社版): 10.3389/fmicb.2018.01555
PubMed ID: 30061871
出現コレクション:学術雑誌掲載論文等

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