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Title: 5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs
Authors: Kaido, Masanori
Nagano, Hideaki
Omote, Kazunori
Takano, Yoshitaka
Mise, Kazuyuki
Okuno, Tetsuro
Author's alias: 海道, 真典
永野, 秀昭
髙野, 義孝
三瀬, 和之
奥野, 哲郎
Keywords: Dianthovirus
Carnation ringspot virus
5′-Terminal stem-loop
Untranslated region
Issue Date: May-2019
Publisher: Elsevier B.V.
Journal title: Virus Research
Volume: 265
Start page: 138
End page: 142
Abstract: Carnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus.
Rights: © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
URI: http://hdl.handle.net/2433/240991
DOI(Published Version): 10.1016/j.virusres.2019.03.009
PubMed ID: 30890436
Appears in Collections:Journal Articles

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