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j.virusres.2019.03.009.pdf | 954.29 kB | Adobe PDF | 見る/開く |
タイトル: | 5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs |
著者: | Kaido, Masanori Nagano, Hideaki Omote, Kazunori Takano, Yoshitaka https://orcid.org/0000-0003-1427-1322 (unconfirmed) Mise, Kazuyuki https://orcid.org/0000-0003-4348-6023 (unconfirmed) Okuno, Tetsuro |
著者名の別形: | 海道, 真典 永野, 秀昭 髙野, 義孝 三瀬, 和之 奥野, 哲郎 |
キーワード: | Dianthovirus Carnation ringspot virus 5′-Terminal stem-loop Untranslated region |
発行日: | May-2019 |
出版者: | Elsevier B.V. |
誌名: | Virus Research |
巻: | 265 |
開始ページ: | 138 |
終了ページ: | 142 |
抄録: | Carnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus. |
著作権等: | © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). |
URI: | http://hdl.handle.net/2433/240991 |
DOI(出版社版): | 10.1016/j.virusres.2019.03.009 |
PubMed ID: | 30890436 |
出現コレクション: | 学術雑誌掲載論文等 |
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