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タイトル: The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams
著者: Masunaga, Shin-ichiro  KAKEN_id
Uzawa, Akiko
Hirayama, Ryoichi
Matsumoto, Yoshitaka
Sakurai, Yoshinori  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-9404-4255 (unconfirmed)
Tanaka, Hiroki
Tano, Keizo  KAKEN_id  orcid https://orcid.org/0000-0003-3900-0035 (unconfirmed)
Sanada, Yu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6264-7983 (unconfirmed)
Suzuki, Minoru  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5421-9417 (unconfirmed)
Maruhashi, Akira
Ono, Koji
著者名の別形: 増永, 慎一郎
真田, 悠生
キーワード: p53 status
Quiescent cell
Carbon-ion beams
Reactor neutron beams
γ-rays
発行日: 2015
出版者: Elmer Press, Inc.
誌名: World Journal of Oncology
巻: 6
号: 4
開始ページ: 398
終了ページ: 409
抄録: Background: The aim of the study was to clarify the effect of p53 status of tumor cells on radiosensitivity of solid tumors following accelerated carbon-ion beam irradiation compared with γ-rays or reactor neutron beams, referring to the response of intratumor quiescent (Q) cells. Methods: Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mp53) or with neo vector (SAS/neo) were injected subcutaneously into hind legs of nude mice. Tumor-bearing mice received 5-bromo-2’-deoxyuridine (BrdU) continuously to label all intratumor proliferating (P) cells. They received γ-rays or accelerated carbon-ion beams at a high or reduced dose-rate. Other tumor-bearing mice received reactor thermal or epithermal neutrons at a reduced dose-rate. Immediately or 9 hours after the high dose-rate irradiation (HDRI), or immediately after the reduced dose-rate irradiation (RDRI), the tumor cells were isolated and incubated with a cytokinesis blocker, and the micronucleus (MN) frequency in cells without BrdU labeling (Q cells) was determined using immunofluorescence staining for BrdU. Results: The difference in radiosensitivity between the total (P + Q) and Q cells after γ-ray irradiation was markedly reduced with reactor neutron beams or carbon-ion beams, especially with a higher linear energy transfer (LET) value. Following γ-ray irradiation, SAS/neo tumor cells, especially intratumor Q cells, showed a marked reduction in sensitivity due to the recovery from radiation-induced damage, compared with the total or Q cells within SAS/mp53 tumors that showed little repair capacity. In both total and Q cells within both SAS/neo and SAS/mp53 tumors, carbon-ion beam irradiation, especially with a higher LET, showed little recovery capacity through leaving an interval between HDRI and the assay or decreasing the dose-rate. The recovery from radiation-induced damage after γ-ray irradiation was a p53-dependent event, but little recovery was found after carbon-ion beam irradiation. With RDRI, the radiosensitivity to reactor thermal and epithermal neutron beams was slightly higher than that to carbon-ion beams. Conclusion: For tumor control, including intratumor Q-cell control, accelerated carbon-ion beams, especially with a higher LET, and reactor thermal and epithermal neutron beams were very useful for suppressing the recovery from radiation-induced damage irrespective of p53 status of tumor cells.
著作権等: © The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/241735
DOI(出版社版): 10.14740/wjon941w
PubMed ID: 28983338
出現コレクション:学術雑誌掲載論文等

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