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タイトル: | Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity |
著者: | Doi, Masao https://orcid.org/0000-0001-6264-9217 (unconfirmed) Shimatani, Hiroyuki Atobe, Yuta Murai, Iori Hayashi, Hida Takahashi, Yukari Fustin, Jean-Michel Yamaguchi, Yoshiaki https://orcid.org/0000-0003-4126-542X (unconfirmed) Kiyonari, Hiroshi Koike, Nobuya Yagita, Kazuhiro Lee, Choogon Abe, Manabu Sakimura, Kenji Okamura, Hitoshi |
著者名の別形: | 土居, 雅夫 嶋谷, 寛之 跡部, 裕太 村井, 伊織 林, 煕達 高橋, ゆかり Fustin, Jean-Michel 山口, 賀章 清成, 寛 小池, 宜也 八木田, 和弘 Lee, Choogon 阿部, 学 崎村, 建司 岡村, 均 |
キーワード: | Circadian rhythms Gene regulation |
発行日: | 12-Jun-2019 |
出版者: | Springer Science and Business Media LLC |
誌名: | Nature Communications |
巻: | 10 |
論文番号: | 2563 |
抄録: | Non-coding cis-regulatory elements are essential determinants of development, but their exact impacts on behavior and physiology in adults remain elusive. Cis-element-based transcriptional regulation is believed to be crucial for generating circadian rhythms in behavior and physiology. However, genetic evidence supporting this model is based on mutations in the protein-coding sequences of clock genes. Here, we report generation of mutant mice carrying a mutation only at the E′-box cis-element in the promoter region of the core clock gene Per2. The Per2 E′-box mutation abolishes sustainable molecular clock oscillations and renders circadian locomotor activity and body temperature rhythms unstable. Without the E′-box, Per2 messenger RNA and protein expression remain at mid-to-high levels. Our work delineates the Per2 E′-box as a critical nodal element for keeping sustainable cell-autonomous circadian oscillation and reveals the extent of the impact of the non-coding cis-element in daily maintenance of animal locomotor activity and body temperature rhythmicity. |
記述: | 体内リズムの発現に必要なDNAのスイッチを発見 --蛋白質をコードしないノンコーディング領域のDNA配列が活躍--. 京都大学プレスリリース. 2019-06-12. |
著作権等: | © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
URI: | http://hdl.handle.net/2433/241740 |
DOI(出版社版): | 10.1038/s41467-019-10532-2 |
PubMed ID: | 31189882 |
関連リンク: | http://www.kyoto-u.ac.jp/ja/research/research_results/2019/190612_1.html |
出現コレクション: | 学術雑誌掲載論文等 |
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