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Title: Three-dimensional live imaging of Atoh1 reveals the dynamics of hair cell induction and organization in the developing cochlea
Authors: Tateya, Tomoko
Sakamoto, Susumu
Ishidate, Fumiyoshi
Hirashima, Tsuyoshi
Imayoshi, Itaru
Kageyama, Ryoichiro
Author's alias: 楯谷, 智子
坂本, 進
石館, 文善
平島, 剛志
今吉, 格
影山, 龍一郎
Keywords: Atoh1
Cochlea
Hair cell
Live imaging
Mouse
Issue Date: 1-Nov-2019
Publisher: The Company of Biologists
Journal title: Development
Volume: 146
Thesis number: dev177881
Abstract: During cochlear development, hair cells (HCs) and supporting cells differentiate in the prosensory domain to form the organ of Corti, but how one row of inner HCs (IHCs) and three rows of outer HCs (OHCs) are organized is not well understood. Here, we investigated the process of HC induction by monitoring Atoh1 expression in cochlear explants of Atoh1-EGFP knock-in mouse embryos and showed that only the cells that express Atoh1 over a certain threshold are selected for HC fate determination. HC induction initially occurs at the medial edge of the prosensory domain to form IHCs and subsequently at the lateral edge to form OHCs, while Hedgehog signaling maintains a space between IHCs and OHCs, leading to formation of the tunnel of Corti. These results reveal dynamic Atoh1 expression in HC fate control and suggest that multi-directional signals regulate OHC induction, thereby organizing the prototype of the organ of Corti.
Rights: © 2019. Published by The Company of Biologists Ltd
The full-text file will be made open to the public on 1 November 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
URI: http://hdl.handle.net/2433/244863
DOI(Published Version): 10.1242/dev.177881
PubMed ID: 31676552
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