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dc.contributor.authorMarcotti, Stefaniaen
dc.contributor.authorMaki, Koichiroen
dc.contributor.authorReilly, Gwendolen C.en
dc.contributor.authorLacroix, Damienen
dc.contributor.authorAdachi, Taijien
dc.contributor.alternative安達, 泰治ja
dc.date.accessioned2019-12-16T07:02:55Z-
dc.date.available2019-12-16T07:02:55Z-
dc.date.issued2018-10-25-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/2433/245139-
dc.description.abstractThe hyaluronic acid component of the glycocalyx plays a role in cell mechanotransduction by selectively transmitting mechanical signals to the cell cytoskeleton or to the cell membrane. The aim of this study was to evaluate the mechanical link between the hyaluronic acid molecule and the cell cytoskeleton by means of atomic force microscopy single molecule force spectroscopy. Hyaluronic acid molecules on live cells were targeted with probes coated with hyaluronic acid binding protein. Two different types of events were observed when the detachment of the target molecule from the probe occurred, suggesting the presence of cytoskeleton- and membrane-anchored molecules. Membrane-anchored molecules facilitated the formation of tethers when pulled. About 15% of the tested hyaluronic acid molecules were shown to be anchored to the cytoskeleton. When multiple molecules bonded to the probe, specific detachment patterns were observed, suggesting that a cytoskeletal bond needed to be broken to improve the ability to pull tethers from the cell membrane. This likely resulted in the formation of tethering structures maintaining a cytoskeletal core similar to the ones observed for cells over-expressing HA synthases. The different observed rupture events were associated with separate mechanotransductive mechanisms in an analogous manner to that previously proposed for the endothelial glycocalyx. Single cytoskeleton anchored rupture events represent HA molecules linked to the cytoskeleton and therefore transmitting mechanical stimuli into the inner cell compartments. Single membrane tethers would conversely represent the glycocalyx molecules connected to areas of the membrane where an abundance of signalling molecules reside.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherPublic Library of Science (PLoS)en
dc.rights© 2018 Marcotti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleHyaluronic acid selective anchoring to the cytoskeleton: An atomic force microscopy studyen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitlePLoS ONEen
dc.identifier.volume13-
dc.identifier.issue10-
dc.relation.doi10.1371/journal.pone.0206056-
dc.textversionpublisher-
dc.identifier.artnume0206056-
dc.addressInsigneo Institute for in silico Medicine, University of Sheffield・Department of Mechanical Engineering, University of Sheffield・Randall Centre for Cell and Molecular Biophysics, King’s College Londonen
dc.addressDepartment of Biosystems Science, Institute for Frontier Life and Medical Sciences, Kyoto University・Department of Mechanical Engineering, University of Tokyoen
dc.addressInsigneo Institute for in silico Medicine, University of Sheffield・Department of Materials Science and Engineering, University of Sheffielden
dc.addressInsigneo Institute for in silico Medicine, University of Sheffield・Department of Mechanical Engineering, University of Sheffielden
dc.addressDepartment of Biosystems Science, Institute for Frontier Life and Medical Sciences, Kyoto Universityen
dc.identifier.pmid30359403-
dcterms.accessRightsopen access-
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