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j.stemcr.2019.07.011.pdf | 23.85 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Kime, Cody | en |
dc.contributor.author | Kiyonari, Hiroshi | en |
dc.contributor.author | Ohtsuka, Satoshi | en |
dc.contributor.author | Kohbayashi, Eiko | en |
dc.contributor.author | Asahi, Michio | en |
dc.contributor.author | Yamanaka, Shinya | en |
dc.contributor.author | Takahashi, Masayo | en |
dc.contributor.author | Tomoda, Kiichiro | en |
dc.contributor.alternative | 清成, 寛 | ja |
dc.contributor.alternative | 大塚, 哲 | ja |
dc.contributor.alternative | 光林, 永子 | ja |
dc.contributor.alternative | 朝日, 通雄 | ja |
dc.contributor.alternative | 山中, 伸弥 | ja |
dc.contributor.alternative | 高橋, 政代 | ja |
dc.contributor.alternative | 友田, 紀一郎 | ja |
dc.date.accessioned | 2020-09-30T01:20:33Z | - |
dc.date.available | 2020-09-30T01:20:33Z | - |
dc.date.issued | 2019-09-10 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/255122 | - |
dc.description.abstract | Soon after fertilization, the few totipotent cells of mammalian embryos diverge to form a structure called the blastocyst (BC). Although numerous cell types, including germ cells and extended-pluripotency stem cells, have been developed from pluripotent stem cells (PSCs) in vitro, generating functional BCs only from PSCs remains elusive. Here, we describe induced self-organizing 3D BC-like cysts (iBLCs) generated from mouse PSC culture. Resembling natural BCs, iBLCs have a blastocoel-like cavity and were formed with outer cells expressing trophectoderm lineage markers and with inner cells expressing pluripotency markers. iBLCs transplanted to pseudopregnant mice uteruses implanted, induced decidualization, and exhibited growth and development before resorption, demonstrating that iBLCs are implantation competent. iBLC precursor intermediates required the transcription factor Prdm14 and concomitantly activated the totipotency-related cleavage-stage MERVL reporter and 2C genes. Thus, our system may contribute to the understanding of molecular mechanisms underpinning totipotency, embryogenesis, and implantation. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | jpn | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2019 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.subject | pluripotent stem cells | en |
dc.subject | early embryo | en |
dc.subject | implantation | en |
dc.subject | cell biology | en |
dc.subject | totipotency | en |
dc.subject | reproduction | en |
dc.subject | cell plasticity | en |
dc.subject | blastocyst | en |
dc.subject | reprogramming | en |
dc.title | Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 485 | - |
dc.identifier.epage | 498 | - |
dc.relation.doi | 10.1016/j.stemcr.2019.07.011 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 31402336 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 17K07246 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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