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タイトル: | Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells |
著者: | Kime, Cody Kiyonari, Hiroshi Ohtsuka, Satoshi Kohbayashi, Eiko Asahi, Michio Yamanaka, Shinya Takahashi, Masayo Tomoda, Kiichiro |
著者名の別形: | 清成, 寛 大塚, 哲 光林, 永子 朝日, 通雄 山中, 伸弥 高橋, 政代 友田, 紀一郎 |
キーワード: | pluripotent stem cells early embryo implantation cell biology totipotency reproduction cell plasticity blastocyst reprogramming |
発行日: | 10-Sep-2019 |
出版者: | Elsevier BV |
誌名: | Stem Cell Reports |
巻: | 13 |
号: | 3 |
開始ページ: | 485 |
終了ページ: | 498 |
抄録: | Soon after fertilization, the few totipotent cells of mammalian embryos diverge to form a structure called the blastocyst (BC). Although numerous cell types, including germ cells and extended-pluripotency stem cells, have been developed from pluripotent stem cells (PSCs) in vitro, generating functional BCs only from PSCs remains elusive. Here, we describe induced self-organizing 3D BC-like cysts (iBLCs) generated from mouse PSC culture. Resembling natural BCs, iBLCs have a blastocoel-like cavity and were formed with outer cells expressing trophectoderm lineage markers and with inner cells expressing pluripotency markers. iBLCs transplanted to pseudopregnant mice uteruses implanted, induced decidualization, and exhibited growth and development before resorption, demonstrating that iBLCs are implantation competent. iBLC precursor intermediates required the transcription factor Prdm14 and concomitantly activated the totipotency-related cleavage-stage MERVL reporter and 2C genes. Thus, our system may contribute to the understanding of molecular mechanisms underpinning totipotency, embryogenesis, and implantation. |
著作権等: | © 2019 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/255122 |
DOI(出版社版): | 10.1016/j.stemcr.2019.07.011 |
PubMed ID: | 31402336 |
出現コレクション: | 学術雑誌掲載論文等 |
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