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dc.contributor.authorSuzuki, Yohei
dc.contributor.authorKano, Kenji
dc.contributor.authorShirai, Osamu
dc.contributor.authorKitazumi, Yuki
dc.contributor.alternative鈴木, 洋平
dc.contributor.alternative加納, 健司
dc.contributor.alternative白井, 理
dc.contributor.alternative北隅, 優希
dc.date.accessioned2020-10-16T05:57:08Z-
dc.date.available2020-10-16T05:57:08Z-
dc.date.issued2020-11-15
dc.identifier.issn1572-6657
dc.identifier.urihttp://hdl.handle.net/2433/255593-
dc.description.abstractAn electrochemical d-fructose sensor was fabricated by immobilizing a variant of d-fructose dehydrogenase on a porous gold microelectrode. The enzyme-modified electrode bioelectrocatalytically oxidizes d-fructose via direct electron transfer. The catalytic current reaches a steady-state limiting value at 0.05 V vs. Ag|AgCl (sat. KCl). The temperature dependence of the sensor suggests that its response is limited by the diffusion of d-fructose. Therefore, the sensor requires no calibration. The sensitivity of the sensor, (2.0 ± 0.2) × 10² μA cm⁻² mM⁻¹, is reproducible. The upper limit of detection is ~2.0 mM and the kinetics of the bioelectrocatalytic reaction is determined the limitation. The fabricated sensors were applied in the determination of d-fructose in commercial beverages as well as honey, and compared favorably with a more commonly used photometric method.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier B.V.
dc.rights© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
dc.rightsThe full-text file will be made open to the public on 15 November 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
dc.subjectd-fructose dehydrogenase
dc.subjectAmperometric biosensor
dc.subjectDirect electron transfer
dc.subjectMicroelectrode
dc.subjectPorous gold
dc.titleDiffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode
dc.type.niitypeJournal Article
dc.identifier.jtitleJournal of Electroanalytical Chemistry
dc.identifier.volume877
dc.relation.doi10.1016/j.jelechem.2020.114651
dc.textversionauthor
dc.identifier.artnum114651
dc.addressDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
dc.addressDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
dc.addressDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
dc.addressDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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