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タイトル: | Diffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode |
著者: | Suzuki, Yohei Kano, Kenji Shirai, Osamu Kitazumi, Yuki |
著者名の別形: | 鈴木, 洋平 加納, 健司 白井, 理 北隅, 優希 |
キーワード: | d-fructose dehydrogenase Amperometric biosensor Direct electron transfer Microelectrode Porous gold |
発行日: | 15-Nov-2020 |
出版者: | Elsevier B.V. |
誌名: | Journal of Electroanalytical Chemistry |
巻: | 877 |
論文番号: | 114651 |
抄録: | An electrochemical d-fructose sensor was fabricated by immobilizing a variant of d-fructose dehydrogenase on a porous gold microelectrode. The enzyme-modified electrode bioelectrocatalytically oxidizes d-fructose via direct electron transfer. The catalytic current reaches a steady-state limiting value at 0.05 V vs. Ag|AgCl (sat. KCl). The temperature dependence of the sensor suggests that its response is limited by the diffusion of d-fructose. Therefore, the sensor requires no calibration. The sensitivity of the sensor, (2.0 ± 0.2) × 10² μA cm⁻² mM⁻¹, is reproducible. The upper limit of detection is ~2.0 mM and the kinetics of the bioelectrocatalytic reaction is determined the limitation. The fabricated sensors were applied in the determination of d-fructose in commercial beverages as well as honey, and compared favorably with a more commonly used photometric method. |
著作権等: | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. The full-text file will be made open to the public on 15 November 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/255593 |
DOI(出版社版): | 10.1016/j.jelechem.2020.114651 |
出現コレクション: | 学術雑誌掲載論文等 |
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