|Title:||Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules.|
Kawano, Kenichi https://orcid.org/0000-0003-1927-2922 (unconfirmed)
Imanishi, Miki https://orcid.org/0000-0002-4172-0966 (unconfirmed)
|Author's alias:||坂本, 健太郎|
|Journal title:||Angewandte Chemie International Edition|
|Abstract:||Endocytic pathways are practical routes for the intracellular delivery of biomacromolecules. Along with this, effective strategies for endosomal cargo release into the cytosol are desired to achieve successful delivery. Focusing on compositional differences between the cell and endosomal membranes and the pH decrease within endosomes, we designed the lipid-sensitive and pH-responsive endosome-lytic peptide HAad. This peptide contains aminoadipic acid (Aad) residues, which serve as a safety catch for preferential permeabilization of endosomal membranes over cell membranes, and His-to-Ala substitutions enhance the endosomolytic activity. The ability of HAad to destabilize endosomal membranes was supported by model studies using large unilamellar vesicles (LUVs) and by increased intracellular delivery of biomacromolecules (including antibodies) into live cells. Cerebral ventricle injection of Cre recombinase with HAad led to Cre/loxP recombination in a mouse model, thus demonstrating potential applicability of HAad in vivo.|
|Rights:||This is the peer reviewed version of the following article: K. Sakamoto, M. Akishiba, T. Iwata, K. Murata, S. Mizuno, K. Kawano, M. Imanishi, F. Sugiyama, S. Futaki, Angew. Chem. Int. Ed. 2020, 59, 19990, which has been published in final form at https://doi.org/10.1002/anie.202005887. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.|
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|Appears in Collections:||Journal Articles|
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