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j.stemcr.2021.03.004.pdf | 4.28 MB | Adobe PDF | 見る/開く |
タイトル: | Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy |
著者: | Nalbandian, Minas Zhao, Mingming Sasaki-Honda, Mitsuru Jonouchi, Tatsuya Lucena-Cacace, Antonio Mizusawa, Takuma Yasuda, Masahiko Yoshida, Yoshinori https://orcid.org/0000-0001-5511-9090 (unconfirmed) Hotta, Akitsu https://orcid.org/0000-0002-2619-7441 (unconfirmed) Sakurai, Hidetoshi https://orcid.org/0000-0002-5383-9366 (unconfirmed) |
著者名の別形: | 趙, 明明 本田, 充 城之内, 達也 水澤, 卓馬 保田, 昌彦 吉田, 善紀 堀田, 秋津 櫻井, 英俊 |
キーワード: | iPSC skeletal muscle muscle stem cell surface marker FGFR4 CDH13 |
発行日: | Apr-2021 |
出版者: | Elsevier BV |
誌名: | Stem Cell Reports |
巻: | 16 |
抄録: | The transplantation of muscle progenitor cells (MuPCs) differentiated from human induced pluripotent stem cells (hiPSCs) is a promising approach for treating skeletal muscle diseases such as Duchenne muscular dystrophy (DMD). However, proper purification of the MuPCs before transplantation is essential for clinical application. Here, by using MYF5 hiPSC reporter lines, we identified two markers for myogenic cell purification: CDH13, which purified most of the myogenic cells, and FGFR4, which purified a subset of MuPCs. Cells purified with each of the markers showed high efficiency for regeneration after transplantation and contributed to the restoration of dystrophin expression in DMD-immunodeficient model mice. Moreover, we found that MYF5 regulates CDH13 expression by binding to the promoter regions. These findings suggest that FGFR4 and CDH13 are strong candidates for the purification of hiPSC-derived MuPCs for therapeutical application. |
記述: | 骨格筋幹細胞を純化する方法を確立 --筋肉の細胞移植治療の実現に向けて--. 京都大学プレスリリース. 2021-04-02. Enhanced muscle regeneration using stem cells. 京都大学プレスリリース. 2021-04-02. |
著作権等: | © 2021 The Authors. This is an open access article under the CC BY-NC-ND license (Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Public License). |
URI: | http://hdl.handle.net/2433/262484 |
DOI(出版社版): | 10.1016/j.stemcr.2021.03.004 |
PubMed ID: | 33798449 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/210402-010000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/210402-010000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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