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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s42003-022-03499-2.pdf | 2.34 MB | Adobe PDF | 見る/開く |
| タイトル: | Cell response analysis in SARS-CoV-2 infected bronchial organoids |
| 著者: | Sano, Emi Suzuki, Tatsuya Hashimoto, Rina Itoh, Yumi Sakamoto, Ayaka Sakai, Yusuke Saito, Akatsuki Okuzaki, Daisuke Motooka, Daisuke Muramoto, Yukiko    https://orcid.org/0000-0002-8706-3113 (unconfirmed)Noda, Takeshi https://orcid.org/0000-0002-0658-4663 (unconfirmed)Takasaki, Tomohiko Sakuragi, Jun-Ichi Minami, Shohei Kobayashi, Takeshi Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed)Matsumura, Yasufumi https://orcid.org/0000-0001-8595-8944 (unconfirmed)Nagao, Miki https://orcid.org/0000-0002-8886-6145 (unconfirmed)Okamoto, Toru Takayama, Kazuo https://orcid.org/0000-0002-1132-2457 (unconfirmed) |
| 著者名の別形: | 佐野, 絵美 鈴木, 達也 橋本, 里菜 伊東, 祐美 坂本, 綾香 坂井, 祐介 齊藤, 暁 奥崎, 大介 元岡, 大祐 村本, 裕紀子 野田, 岳志 髙崎, 智彦 櫻木, 淳一 南, 昌平 小林, 剛 山本, 拓也 松村, 康史 長尾, 美紀 岡本, 徹 高山, 和雄 |
| キーワード: | Respiratory tract diseases SARS-CoV-2 |
| 発行日: | 2022 |
| 出版者: | Springer Nature |
| 誌名: | Communications Biology |
| 巻: | 5 |
| 論文番号: | 516 |
| 抄録: | The development of an in vitro cell model that can be used to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research is expected. Here we conducted infection experiments in bronchial organoids (BO) and an BO-derived air-liquid interface model (BO-ALI) using 8 SARS-CoV-2 variants. The infection efficiency in BO-ALI was more than 1, 000 times higher than that in BO. Among the bronchial epithelial cells, we found that ciliated cells were infected with the virus, but basal cells were not. Ciliated cells died 7 days after the viral infection, but basal cells survived after the viral infection and differentiated into ciliated cells. Fibroblast growth factor 10 signaling was essential for this differentiation. These results indicate that BO and BO-ALI may be used not only to evaluate the cell response to SARS-CoV-2 and coronavirus disease 2019 (COVID-19) therapeutic agents, but also for airway regeneration studies. |
| 記述: | 気管支オルガノイドを用いた新型コロナウイルス研究とその創薬応用. 京都大学プレスリリース. 2022-05-30. COVID-19 Research Using Bronchial Organoids and Drug Discovery Applications. 京都大学プレスリリース. 2022-06-02. |
| 著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| URI: | http://hdl.handle.net/2433/274182 |
| DOI(出版社版): | 10.1038/s42003-022-03499-2 |
| PubMed ID: | 35637255 |
| 関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220530-180000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/220602-170000.html |
| 出現コレクション: | 学術雑誌掲載論文等 |
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