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| File | Description | Size | Format | |
|---|---|---|---|---|
| j.xpro.2021.100967.pdf | 5.41 MB | Adobe PDF | View/Open |
| Title: | Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy |
| Authors: | Miyoshi, Takushi Friedman, Thomas B. Watanabe, Naoki    https://orcid.org/0000-0001-8492-200X (unconfirmed) |
| Author's alias: | 三好, 拓志 渡邊, 直樹 |
| Keywords: | Single-molecule Assays High Throughput Screening Microscopy Antibody Molecular/Chemical Probes Protein Biochemistry |
| Issue Date: | Dec-2021 |
| Publisher: | Elsevier BV |
| Journal title: | STAR Protocols |
| Volume: | 2 |
| Issue: | 4 |
| Thesis number: | 100967 |
| Abstract: | Fast-dissociating, highly specific monoclonal antibodies (FDSAs) are single-molecule imaging probes useful for many biological assays including consecutive, multiplexable super-resolution microscopy. We developed a screening assay to characterize the kinetics of antibody-antigen interactions using single-molecule microscopy and established a pipeline to identify FDSAs from thousands of monoclonal candidates. Provided here are detailed protocols to prepare multi-well glass-bottom plates necessary for our assay to identify hybridoma clones secreting FDSAs. Synthesis of fluorescently labeled Fab fragments (Fab probes) from FDSAs is also described. |
| Rights: | This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
| URI: | http://hdl.handle.net/2433/274201 |
| DOI(Published Version): | 10.1016/j.xpro.2021.100967 |
| PubMed ID: | 34841279 |
| Appears in Collections: | Journal Articles |
This item is licensed under a Creative Commons License
