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このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.xpro.2021.100967.pdf | 5.41 MB | Adobe PDF | 見る/開く |
| タイトル: | Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy |
| 著者: | Miyoshi, Takushi Friedman, Thomas B. Watanabe, Naoki    https://orcid.org/0000-0001-8492-200X (unconfirmed) |
| 著者名の別形: | 三好, 拓志 渡邊, 直樹 |
| キーワード: | Single-molecule Assays High Throughput Screening Microscopy Antibody Molecular/Chemical Probes Protein Biochemistry |
| 発行日: | Dec-2021 |
| 出版者: | Elsevier BV |
| 誌名: | STAR Protocols |
| 巻: | 2 |
| 号: | 4 |
| 論文番号: | 100967 |
| 抄録: | Fast-dissociating, highly specific monoclonal antibodies (FDSAs) are single-molecule imaging probes useful for many biological assays including consecutive, multiplexable super-resolution microscopy. We developed a screening assay to characterize the kinetics of antibody-antigen interactions using single-molecule microscopy and established a pipeline to identify FDSAs from thousands of monoclonal candidates. Provided here are detailed protocols to prepare multi-well glass-bottom plates necessary for our assay to identify hybridoma clones secreting FDSAs. Synthesis of fluorescently labeled Fab fragments (Fab probes) from FDSAs is also described. |
| 著作権等: | This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
| URI: | http://hdl.handle.net/2433/274201 |
| DOI(出版社版): | 10.1016/j.xpro.2021.100967 |
| PubMed ID: | 34841279 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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