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dc.contributor.author | Fujiwara, Naoko | en |
dc.contributor.author | Shigemoto, Maki | en |
dc.contributor.author | Hirayama, Mizuki | en |
dc.contributor.author | Fujita, Ken-ichi | en |
dc.contributor.author | Seno, Shigeto | en |
dc.contributor.author | Matsuda, Hideo | en |
dc.contributor.author | Nagahama, Masami | en |
dc.contributor.author | Masuda, Seiji | en |
dc.contributor.alternative | 藤原, 奈央子 | ja |
dc.contributor.alternative | 重本, 真紀 | ja |
dc.contributor.alternative | 平山, 瑞季 | ja |
dc.contributor.alternative | 藤田, 賢一 | ja |
dc.contributor.alternative | 瀬尾, 茂人 | ja |
dc.contributor.alternative | 松田, 秀雄 | ja |
dc.contributor.alternative | 長浜, 正巳 | ja |
dc.contributor.alternative | 増田, 誠司 | ja |
dc.date.accessioned | 2022-09-01T01:48:04Z | - |
dc.date.available | 2022-09-01T01:48:04Z | - |
dc.date.issued | 2022-08-26 | - |
dc.identifier.uri | http://hdl.handle.net/2433/276061 | - |
dc.description | ヒト細胞内でRNA分解時に働く因子の役割を解明 --細胞内におけるRNA分解機構の全容解明に期待--. 京都大学プレスリリース. 2022-08-05. | ja |
dc.description.abstract | Recent in vitro reconstitution analyses have proven that the physical interaction between the exosome core and MTR4 helicase, which promotes the exosome activity, is maintained by either MPP6 or RRP6. However, knowledge regarding the function of MPP6 with respect to in vivo exosome activity remains scarce. Here, we demonstrate a facilitative function of MPP6 that composes a specific part of MTR4-dependent substrate decay by the human exosome. Using RNA polymerase II-transcribed poly(A)⁺ substrate accumulation as an indicator of a perturbed exosome, we found functional redundancy between RRP6 and MPP6 in the decay of these poly(A)⁺ transcripts. MTR4 binding to the exosome core via MPP6 was essential for MPP6 to exert its redundancy with RRP6. However, at least for the decay of our identified exosome substrates, MTR4 recruitment by MPP6 was not functionally equivalent to recruitment by RRP6. Genome-wide classification of substrates based on their sensitivity to each exosome component revealed that MPP6 deals with a specific range of substrates and highlights the importance of MTR4 for their decay. Considering recent findings of competitive binding to the exosome between auxiliary complexes, our results suggest that the MPP6-incorporated MTR4-exosome complex is one of the multiple alternative complexes rather than the prevailing one. | en |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press (OUP) | en |
dc.publisher | Nucleic Acids Research | en |
dc.rights | © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. | en |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | RNA and RNA-protein complexes | en |
dc.title | MPP6 stimulates both RRP6 and DIS3 to degrade a specified subset of MTR4-sensitive substrates in the human nucleus | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nucleic Acids Research | en |
dc.identifier.volume | 50 | - |
dc.identifier.issue | 15 | - |
dc.identifier.spage | 8779 | - |
dc.identifier.epage | 8806 | - |
dc.relation.doi | 10.1093/nar/gkac559 | - |
dc.textversion | publisher | - |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Graduate School of Biostudies, Kyoto University | en |
dc.address | Graduate School of Biostudies, Kyoto University; Division of Gene Expression Mechanism, Institute for Comprehensive Medical Science, Fujita Health University | en |
dc.address | Graduate School of Information Science and Technology, Osaka University | en |
dc.address | Graduate School of Information Science and Technology, Osaka University | en |
dc.address | Laboratory of Molecular and Cellular Biochemistry, Meiji Pharmaceutical University | en |
dc.address | Graduate School of Biostudies, Kyoto University; Department of Food Science and Nutrition, Faculty of Agriculture Kindai University; Agricultural Technology and Innovation Research Institute, Kindai University; Antiaging center, Kindai University | en |
dc.identifier.pmid | 35902094 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2022-08-05 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 21K19078 | - |
datacite.awardNumber | 19H02884 | - |
datacite.awardNumber | 19K22280 | - |
datacite.awardNumber | 26292053 | - |
datacite.awardNumber | 23658289 | - |
datacite.awardNumber | 21K19078 | - |
datacite.awardNumber | 22H02264 | - |
datacite.awardNumber | 16H06279 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19078/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H02884/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22280/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26292053/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23658289/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19078/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02264/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H06279/ | - |
dc.identifier.pissn | 0305-1048 | - |
dc.identifier.eissn | 1362-4962 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | スプライシング阻害活性をモデルとした食品化合物の迅速探索・評価法の開発と応用展開 | ja |
jpcoar.awardTitle | 真核細胞におけるmRNA核外輸送体の分子進化による輸送体多様化の分子基盤の解明 | ja |
jpcoar.awardTitle | 活性フラボノイドによる選択的mRNAスプライシング制御の分子機構解明と応用展開 | ja |
jpcoar.awardTitle | 真核細胞におけるmRNA輸送基盤の解明とアプライドmRNAバイオテクロジー | ja |
jpcoar.awardTitle | 次世代シークエンサによる細胞核mRNA動態解析基盤と品質管理因子の作用機序の解析 | ja |
jpcoar.awardTitle | スプライシング阻害活性をモデルとした食品化合物の迅速探索・評価法の開発と応用展開 | ja |
jpcoar.awardTitle | ヒトにおける核内mRNA輸送経路の多様化とその生理的意義の解明 | ja |
jpcoar.awardTitle | 先進ゲノム解析研究推進プラットフォーム | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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