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タイトル: | MPP6 stimulates both RRP6 and DIS3 to degrade a specified subset of MTR4-sensitive substrates in the human nucleus |
著者: | Fujiwara, Naoko Shigemoto, Maki Hirayama, Mizuki Fujita, Ken-ichi Seno, Shigeto Matsuda, Hideo Nagahama, Masami Masuda, Seiji |
著者名の別形: | 藤原, 奈央子 重本, 真紀 平山, 瑞季 藤田, 賢一 瀬尾, 茂人 松田, 秀雄 長浜, 正巳 増田, 誠司 |
キーワード: | RNA and RNA-protein complexes |
発行日: | 26-Aug-2022 |
出版者: | Oxford University Press (OUP) Nucleic Acids Research |
誌名: | Nucleic Acids Research |
巻: | 50 |
号: | 15 |
開始ページ: | 8779 |
終了ページ: | 8806 |
抄録: | Recent in vitro reconstitution analyses have proven that the physical interaction between the exosome core and MTR4 helicase, which promotes the exosome activity, is maintained by either MPP6 or RRP6. However, knowledge regarding the function of MPP6 with respect to in vivo exosome activity remains scarce. Here, we demonstrate a facilitative function of MPP6 that composes a specific part of MTR4-dependent substrate decay by the human exosome. Using RNA polymerase II-transcribed poly(A)⁺ substrate accumulation as an indicator of a perturbed exosome, we found functional redundancy between RRP6 and MPP6 in the decay of these poly(A)⁺ transcripts. MTR4 binding to the exosome core via MPP6 was essential for MPP6 to exert its redundancy with RRP6. However, at least for the decay of our identified exosome substrates, MTR4 recruitment by MPP6 was not functionally equivalent to recruitment by RRP6. Genome-wide classification of substrates based on their sensitivity to each exosome component revealed that MPP6 deals with a specific range of substrates and highlights the importance of MTR4 for their decay. Considering recent findings of competitive binding to the exosome between auxiliary complexes, our results suggest that the MPP6-incorporated MTR4-exosome complex is one of the multiple alternative complexes rather than the prevailing one. |
記述: | ヒト細胞内でRNA分解時に働く因子の役割を解明 --細胞内におけるRNA分解機構の全容解明に期待--. 京都大学プレスリリース. 2022-08-05. |
著作権等: | © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/276061 |
DOI(出版社版): | 10.1093/nar/gkac559 |
PubMed ID: | 35902094 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-08-05 |
出現コレクション: | 学術雑誌掲載論文等 |
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