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j.celrep.2022.111323.pdf | 3.12 MB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Suno, Ryoji | en |
dc.contributor.author | Sugita, Yukihiko | en |
dc.contributor.author | Morimoto, Kazushi | en |
dc.contributor.author | Takazaki, Hiroko | en |
dc.contributor.author | Tsujimoto, Hirokazu | en |
dc.contributor.author | Hirose, Mika | en |
dc.contributor.author | Suno-Ikeda, Chiyo | en |
dc.contributor.author | Nomura, Norimichi | en |
dc.contributor.author | Hino, Tomoya | en |
dc.contributor.author | Inoue, Asuka | en |
dc.contributor.author | Iwasaki, Kenji | en |
dc.contributor.author | Kato, Takayuki | en |
dc.contributor.author | Iwata, So | en |
dc.contributor.author | Kobayashi, Takuya | en |
dc.contributor.alternative | 寿野, 良二 | ja |
dc.contributor.alternative | 杉田, 征彦 | ja |
dc.contributor.alternative | 森本, 和志 | ja |
dc.contributor.alternative | 髙﨑, 寛子 | ja |
dc.contributor.alternative | 辻本, 浩一 | ja |
dc.contributor.alternative | 廣瀬, 未果 | ja |
dc.contributor.alternative | 寿野, 千代 | ja |
dc.contributor.alternative | 野村, 紀通 | ja |
dc.contributor.alternative | 日野, 智也 | ja |
dc.contributor.alternative | 井上, 飛鳥 | ja |
dc.contributor.alternative | 岩崎, 憲治 | ja |
dc.contributor.alternative | 加藤, 貴之 | ja |
dc.contributor.alternative | 岩田, 想 | ja |
dc.contributor.alternative | 清水(小林), 拓也 | ja |
dc.date.accessioned | 2022-09-15T08:32:36Z | - |
dc.date.available | 2022-09-15T08:32:36Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.uri | http://hdl.handle.net/2433/276290 | - |
dc.description | 熱、炎症などに関与するプロスタグランジン受容体EP3シグナリング複合体の可視化 --緑内障、高眼圧症治療薬の合理的設計に貢献--. 京都大学プレスリリース. 2022-09-15. | ja |
dc.description.abstract | Prostaglandin receptors have been implicated in a wide range of functions, including inflammation, immune response, reproduction, and cancer. Our group has previously determined the crystal structure of the active-like EP3 bound to its endogenous agonist, prostaglandin E₂. Here, we present the single-particle cryoelectron microscopy (cryo-EM) structure of the human EP3-Gi signaling complex at a resolution of 3.4 Å. The structure reveals the binding mode of Gi to EP3 and the structural changes induced in EP3 by Gi binding. In addition, we compare the structure of the EP3-Gi complex with other subtypes of prostaglandin receptors (EP2 and EP4) bound to Gs that have been previously reported and examine the differences in amino acid composition at the receptor-G protein interface. Mutational analysis reveals that the selectivity of the G protein depends on specific amino acid residues in the second intracellular loop and TM5. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2022 The Author(s). | en |
dc.rights | This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | G protein-coupled receptor | en |
dc.subject | prostaglandin receptor | en |
dc.subject | cryoelectron microscopy single-particle analysis | en |
dc.subject | signal transduction | en |
dc.subject | G protein-coupling selectivity | en |
dc.title | Structural insights into the G protein selectivity revealed by the human EP3-Gi signaling complex | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cell Reports | en |
dc.identifier.volume | 40 | - |
dc.identifier.issue | 11 | - |
dc.relation.doi | 10.1016/j.celrep.2022.111323 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 111323 | - |
dc.address | Department of Medical Chemistry, Kansai Medical University | en |
dc.address | Institute for Protein Research, Osaka University; Hakubi Center for Advanced Research, Kyoto University; Institute for Life and Medical Sciences, Kyoto University | en |
dc.address | Physical Chemistry for Life Science Laboratory, Faculty of Pharmaceutical Sciences, Kyushu University | en |
dc.address | Institute for Protein Research, Osaka University | en |
dc.address | Department of Cell Biology and Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | Institute for Protein Research, Osaka University | en |
dc.address | Department of Medical Chemistry, Kansai Medical University | en |
dc.address | Department of Cell Biology and Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University; Center for Research on Green Sustainable Chemistry, Tottori University | en |
dc.address | Graduate School of Pharmaceutical Sciences, Tohoku University | en |
dc.address | Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba | en |
dc.address | Institute for Protein Research, Osaka University | en |
dc.address | Department of Cell Biology and Medical Chemistry, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Medical Chemistry, Kansai Medical University; Japan Agency for Medical Research and Development (AMED), Core Research for Evolutional Science and Technology (CREST) | en |
dc.identifier.pmid | 36103815 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2022-09-15 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 15K08268 | - |
datacite.awardNumber | 19H03428 | - |
datacite.awardNumber | 20H03434 | - |
datacite.awardNumber | 21H04791 | - |
datacite.awardNumber | 21H05113 | - |
datacite.awardNumber | 21H05112 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K08268/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03428/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H03434/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H04791/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21H05113/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21H05112/ | - |
dc.identifier.eissn | 2211-1247 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 睡眠制御薬の合理的開発に資する活性型/不活性型オレキシン受容体結晶構造の差分解析 | ja |
jpcoar.awardTitle | バイアスリガンド開発に資するG蛋白質/アレスチン-GPCR複合体の構造解析 | ja |
jpcoar.awardTitle | プロスタグランジン受容体を標的とした構造に基づく創薬 | ja |
jpcoar.awardTitle | G12/13シグナルを標的としたデザイナーGPCRの開発と疾患治療戦略 | ja |
jpcoar.awardTitle | オピオイド受容体の網羅的シグナル解析による薬理経路の同定 | ja |
jpcoar.awardTitle | 過渡的タンパク質複合体の高速構造解析プラットフォームの構築 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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