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Title: Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds
Authors: Hippler, Marc
Weißenbruch, Kai
Richler, Kai
Lemma, Enrico D.
Nakahata, Masaki
Richter, Benjamin
Barner-Kowollik, Christopher
Takashima, Yoshinori
Harada, Akira
Blasco, Eva
Wegener, Martin
Tanaka, Motomu  kyouindb  KAKEN_id  orcid (unconfirmed)
Bastmeyer, Martin
Author's alias: 田中, 求
Issue Date: Sep-2020
Publisher: American Association for the Advancement of Science (AAAS)
Journal title: Science Advances
Volume: 6
Issue: 39
Thesis number: eabc2648
Abstract: Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors.
Rights: © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
DOI(Published Version): 10.1126/sciadv.abc2648
PubMed ID: 32967835
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