ダウンロード数: 51
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
sciadv.abc2648.pdf | 5.03 MB | Adobe PDF | 見る/開く |
タイトル: | Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds |
著者: | Hippler, Marc Weißenbruch, Kai Richler, Kai Lemma, Enrico D. Nakahata, Masaki Richter, Benjamin Barner-Kowollik, Christopher Takashima, Yoshinori Harada, Akira Blasco, Eva Wegener, Martin Tanaka, Motomu ![]() ![]() Bastmeyer, Martin |
著者名の別形: | 田中, 求 |
発行日: | Sep-2020 |
出版者: | American Association for the Advancement of Science (AAAS) |
誌名: | Science Advances |
巻: | 6 |
号: | 39 |
論文番号: | eabc2648 |
抄録: | Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors. |
著作権等: | © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/276315 |
DOI(出版社版): | 10.1126/sciadv.abc2648 |
PubMed ID: | 32967835 |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス