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タイトル: Diversity analysis of thermophilic hydrogenogenic carboxydotrophs by carbon monoxide dehydrogenase amplicon sequencing using new primers
著者: Omae, Kimiho
Oguro, Tatsuki
Inoue, Masao
Fukuyama, Yuto
Yoshida, Takashi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5257-0617 (unconfirmed)
Sako, Yoshihiko
著者名の別形: 大前, 公保
大黒, 達希
井上, 真男
福山, 宥斗
吉田, 天士
左子, 芳彦
キーワード: Carbon monoxide dehydrogenase
Carboxydotroph
Hydrogen production
Group 4 [NiFe] hydrogenase
Thermophile
Amplicon sequencing
Rare biosphere
発行日: Jan-2021
出版者: Springer Nature
誌名: Extremophiles
巻: 25
号: 1
開始ページ: 61
終了ページ: 76
抄録: The microbial H2-producing (hydrogenogenic) carbon monoxide (CO)-oxidizing activity by the membrane-associated CO dehydrogenase (CODH)/energy-converting hydrogenase (ECH) complex is an important metabolic process in the microbial community. However, the studies on hydrogenogenic carboxydotrophs had to rely on inherently cultivation and isolation methods due to their rare abundance, which was a bottleneck in ecological study. Here, we provided gene-targeted sequencing method for the diversity estimation of thermophilic hydrogenogenic carboxydotrophs. We designed six new degenerate primer pairs which effectively amplified the coding regions of CODH genes forming gene clusters with ECH genes (CODHech genes) in Firmicutes which includes major thermophilic hydrogenogenic carboxydotrophs in terrestrial thermal habitats. Amplicon sequencing by these primers using DNAs from terrestrial hydrothermal sediments and CO-gas-incubated samples specifically detected multiple CODH genes which were identical or phylogenetically related to the CODHech genes in Firmictes. Furthermore, we found that phylogenetically distinct CODHech genes were enriched in CO-gas-incubated samples, suggesting that our primers detected uncultured hydrogenogenic carboxydotrophs as well. The new CODH-targeted primers provided us with a fine-grained (~ 97.9% in nucleotide sequence identity) diversity analysis of thermophilic hydrogenogenic carboxydotrophs by amplicon sequencing and will bolster the ecological study of these microorganisms.
著作権等: © The Author(s) 2021
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
URI: http://hdl.handle.net/2433/276520
DOI(出版社版): 10.1007/s00792-020-01211-y
PubMed ID: 33415441
出現コレクション:学術雑誌掲載論文等

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