ダウンロード数: 63
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
acschembio.2c00221.pdf | 1.7 MB | Adobe PDF | 見る/開く |
タイトル: | Chemical Probe-Based Nanopore Sequencing to Selectively Assess the RNA Modifications |
著者: | Ramasamy, Soundhar Sahayasheela, Vinodh J. Sharma, Surbhi Yu, Zutao Hidaka, Takuya Cai, Li Thangavel, Vaijayanthi Sugiyama, Hiroshi Pandian, Ganesh N. |
著者名の別形: | 余, 祖滔 日髙, 拓也 杉山, 弘 |
キーワード: | Genetics Mathematical methods Modification Reactivity Rodent models |
発行日: | 21-Oct-2022 |
出版者: | American Chemical Society (ACS) |
誌名: | ACS Chemical Biology |
巻: | 17 |
号: | 10 |
開始ページ: | 2704 |
終了ページ: | 2709 |
抄録: | Nanopore direct RNA sequencing (dRNA-Seq) reads reveal RNA modifications through consistent error profiles specific to a modified nucleobase. However, a null data set is required to identify actual RNA modification-associated errors for distinguishing it from confounding highly intrinsic sequencing errors. Here, we reveal that inosine creates a signature mismatch error in dRNA-Seq reads and obviates the need for a null data set by harnessing the selective reactivity of acrylonitrile for validating the presence of actual inosine modifications. Selective reactivity of acrylonitrile toward inosine altered multiple dRNA-Seq parameters like signal intensity and trace value. We also deduced the stoichiometry of inosine modification through deviation in signal intensity and trace value using this chemical biology approach. Furthermore, we devised Nano ICE-Seq, a protocol to overcome the low coverage issue associated with direct RNA sequencing. Taken together, our chemical probe-based approach may facilitate the knockout-free detection of disease-associated RNA modifications in clinical scenarios. |
著作権等: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in 'ACS Chemical Biology', copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acschembio.2c00221. The full-text file will be made open to the public on 3 October 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/277273 |
DOI(出版社版): | 10.1021/acschembio.2c00221 |
PubMed ID: | 36190780 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。