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Title: Improved Sendai viral system for reprogramming to naive pluripotency
Authors: Kunitomi, Akira
Hirohata, Ryoko
Arreola, Vanessa
Osawa, Mitsujiro
Kato, Tomoaki M.
Nomura, Masaki
Kawaguchi, Jitsutaro
Hara, Hiroto
Kusano, Kohji
Takashima, Yasuhiro
Takahashi, Kazutoshi
Fukuda, Keiichi
Takasu, Naoko
Yamanaka, Shinya
Author's alias: 國富, 晃
廣畑, 糧子
大澤, 光次郎
加藤, 智朗
野村, 真樹
川口, 実太郎
原, 裕人
草野, 好司
髙島, 康弘
高橋, 和利
福田, 恵一
高須, 直子
山中, 伸弥
Keywords: Sendai virus vector
reprogramming
naive pluripotency
induced pluripotent stem cells
residual transgenes
temperature sensitivity
LMYC
hsa-microRNA-367
feeder-free culture
extra-embryonic trophectoderm
Issue Date: 21-Nov-2022
Publisher: Elsevier BV
Journal title: Cell Reports Methods
Volume: 2
Issue: 11
Thesis number: 100317
Abstract: Naive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified method can be applied not only to fibroblasts but also to other somatic cell types. SeV vectors disappear quickly at early passages, and this approach enables the generation of naive iPSCs in a feeder-free culture. The naive iPSCs generated by this method show better differentiation to trilineage and extra-embryonic trophectoderm than those derived by conventional methods. This method can expand the application of iPSCs to research on early human development and regenerative medicine.
Description: 優れた多分化能を持つヒトのナイーブ型iPS細胞を迅速に作製する方法を発明. 京都大学プレスリリース. 2022-10-18.
A novel method for generating naive human iPS cells with significantly higher differentiation potency. 京都大学プレスリリース. 2022-11-15.
Rights: © 2022 The Authors.
This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license.
URI: http://hdl.handle.net/2433/277473
DOI(Published Version): 10.1016/j.crmeth.2022.100317
Related Link: https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/221018-000000.html
https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/221115-100000.html
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