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dc.contributor.authorKunitomi, Akiraen
dc.contributor.authorHirohata, Ryokoen
dc.contributor.authorArreola, Vanessaen
dc.contributor.authorOsawa, Mitsujiroen
dc.contributor.authorKato, Tomoaki M.en
dc.contributor.authorNomura, Masakien
dc.contributor.authorKawaguchi, Jitsutaroen
dc.contributor.authorHara, Hirotoen
dc.contributor.authorKusano, Kohjien
dc.contributor.authorTakashima, Yasuhiroen
dc.contributor.authorTakahashi, Kazutoshien
dc.contributor.authorFukuda, Keiichien
dc.contributor.authorTakasu, Naokoen
dc.contributor.authorYamanaka, Shinyaen
dc.contributor.alternative國富, 晃ja
dc.contributor.alternative廣畑, 糧子ja
dc.contributor.alternative大澤, 光次郎ja
dc.contributor.alternative加藤, 智朗ja
dc.contributor.alternative野村, 真樹ja
dc.contributor.alternative川口, 実太郎ja
dc.contributor.alternative原, 裕人ja
dc.contributor.alternative草野, 好司ja
dc.contributor.alternative髙島, 康弘ja
dc.contributor.alternative高橋, 和利ja
dc.contributor.alternative福田, 恵一ja
dc.contributor.alternative高須, 直子ja
dc.contributor.alternative山中, 伸弥ja
dc.date.accessioned2022-11-24T07:08:15Z-
dc.date.available2022-11-24T07:08:15Z-
dc.date.issued2022-11-21-
dc.identifier.urihttp://hdl.handle.net/2433/277473-
dc.description優れた多分化能を持つヒトのナイーブ型iPS細胞を迅速に作製する方法を発明. 京都大学プレスリリース. 2022-10-18.ja
dc.descriptionA novel method for generating naive human iPS cells with significantly higher differentiation potency. 京都大学プレスリリース. 2022-11-15.en
dc.description.abstractNaive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified method can be applied not only to fibroblasts but also to other somatic cell types. SeV vectors disappear quickly at early passages, and this approach enables the generation of naive iPSCs in a feeder-free culture. The naive iPSCs generated by this method show better differentiation to trilineage and extra-embryonic trophectoderm than those derived by conventional methods. This method can expand the application of iPSCs to research on early human development and regenerative medicine.en
dc.language.isoeng-
dc.publisherElsevier BVen
dc.rights© 2022 The Authors.en
dc.rightsThis is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subjectSendai virus vectoren
dc.subjectreprogrammingen
dc.subjectnaive pluripotencyen
dc.subjectinduced pluripotent stem cellsen
dc.subjectresidual transgenesen
dc.subjecttemperature sensitivityen
dc.subjectLMYCen
dc.subjecthsa-microRNA-367en
dc.subjectfeeder-free cultureen
dc.subjectextra-embryonic trophectodermen
dc.titleImproved Sendai viral system for reprogramming to naive pluripotencyen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleCell Reports Methodsen
dc.identifier.volume2-
dc.identifier.issue11-
dc.relation.doi10.1016/j.crmeth.2022.100317-
dc.textversionpublisher-
dc.identifier.artnum100317-
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; Gladstone Institute of Cardiovascular Diseaseen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; CiRA Foundationen
dc.addressGladstone Institute of Cardiovascular Diseaseen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; Present address: Thyas Co., Ltd.en
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; CiRA Foundationen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; CiRA Foundationen
dc.addressID Pharma Co., Ltd.en
dc.addressID Pharma Co., Ltd.en
dc.addressID Pharma Co., Ltd.en
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto Universityen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto Universityen
dc.addressDepartment of Cardiology, Keio University School of Medicineen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; CiRA Foundationen
dc.addressCenter for iPS Cell Research and Application (CiRA), Kyoto University; Gladstone Institute of Cardiovascular Disease; CiRA Foundation; Department of Anatomy, University of California San Franciscoen
dc.identifier.pmid36447645-
dc.relation.urlhttps://www.cira.kyoto-u.ac.jp/j/pressrelease/news/221018-000000.html-
dc.relation.urlhttps://www.cira.kyoto-u.ac.jp/e/pressrelease/news/221115-100000.html-
dcterms.accessRightsopen access-
datacite.awardNumber16K19429-
datacite.awardNumber18K15846-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K19429/-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K15846/-
dc.identifier.eissn2667-2375-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.awardTitleiPS細胞の質を改善する新規樹立方法の開発ja
jpcoar.awardTitle高品質ヒトiPS細胞を用いた成熟心筋分化誘導法の確立ja
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