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j.crmeth.2022.100317.pdf | 3.24 MB | Adobe PDF | 見る/開く |
タイトル: | Improved Sendai viral system for reprogramming to naive pluripotency |
著者: | Kunitomi, Akira Hirohata, Ryoko Arreola, Vanessa Osawa, Mitsujiro Kato, Tomoaki M. Nomura, Masaki Kawaguchi, Jitsutaro Hara, Hiroto Kusano, Kohji Takashima, Yasuhiro https://orcid.org/0000-0003-0126-7260 (unconfirmed) Takahashi, Kazutoshi Fukuda, Keiichi Takasu, Naoko Yamanaka, Shinya |
著者名の別形: | 國富, 晃 廣畑, 糧子 大澤, 光次郎 加藤, 智朗 野村, 真樹 川口, 実太郎 原, 裕人 草野, 好司 髙島, 康弘 高橋, 和利 福田, 恵一 高須, 直子 山中, 伸弥 |
キーワード: | Sendai virus vector reprogramming naive pluripotency induced pluripotent stem cells residual transgenes temperature sensitivity LMYC hsa-microRNA-367 feeder-free culture extra-embryonic trophectoderm |
発行日: | 21-Nov-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports Methods |
巻: | 2 |
号: | 11 |
論文番号: | 100317 |
抄録: | Naive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified method can be applied not only to fibroblasts but also to other somatic cell types. SeV vectors disappear quickly at early passages, and this approach enables the generation of naive iPSCs in a feeder-free culture. The naive iPSCs generated by this method show better differentiation to trilineage and extra-embryonic trophectoderm than those derived by conventional methods. This method can expand the application of iPSCs to research on early human development and regenerative medicine. |
記述: | 優れた多分化能を持つヒトのナイーブ型iPS細胞を迅速に作製する方法を発明. 京都大学プレスリリース. 2022-10-18. A novel method for generating naive human iPS cells with significantly higher differentiation potency. 京都大学プレスリリース. 2022-11-15. |
著作権等: | © 2022 The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/277473 |
DOI(出版社版): | 10.1016/j.crmeth.2022.100317 |
PubMed ID: | 36447645 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/221018-000000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/221115-100000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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