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タイトル: | Shedding light on the base-pair opening dynamics of nucleic acids in living human cells |
著者: | Yamaoki, Yudai https://orcid.org/0000-0002-8927-4985 (unconfirmed) Nagata, Takashi https://orcid.org/0000-0002-3733-2709 (unconfirmed) Kondo, Keiko https://orcid.org/0000-0003-2860-2751 (unconfirmed) Sakamoto, Tomoki Takami, Shohei Katahira, Masato https://orcid.org/0000-0003-0336-7660 (unconfirmed) |
著者名の別形: | 山置, 佑大 永田, 崇 近藤, 敬子 阪本, 知樹 高見, 昇平 片平, 正人 |
キーワード: | Nucleic acids Solution-state NMR |
発行日: | 29-Nov-2022 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 13 |
論文番号: | 7143 |
抄録: | Base-pair opening is a fundamental property of nucleic acids that plays important roles in biological functions. However, studying the base-pair opening dynamics inside living cells has remained challenging. Here, to determine the base-pair opening kinetics inside living human cells, the exchange rate constant (kex) of the imino proton with the proton of solvent water involved in hairpin and G-quadruplex (GQ) structures is determined by the in-cell NMR technique. It is deduced on determination of kex values that at least some G-C base pairs of the hairpin structure and all G-G base-pairs of the GQ structure open more frequently in living human cells than in vitro. It is suggested that interactions with endogenous proteins could be responsible for the increase in frequency of base-pair opening. Our studies demonstrate a difference in dynamics of nucleic acids between in-cell and in vitro conditions. |
記述: | 生細胞中の核酸のダイナミクスに光を当てる --生細胞中における挙動は試験管中とは異なる--. 京都大学プレスリリース. 2022-11-30. |
著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/277589 |
DOI(出版社版): | 10.1038/s41467-022-34822-4 |
PubMed ID: | 36446768 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-11-30 |
出現コレクション: | 学術雑誌掲載論文等 |
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