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タイトル: | Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin |
著者: | Yamashiro, Sawako ![]() ![]() ![]() Rutkowski, David M. Lynch, Kelli Ann Liu, Ying Vavylonis, Dimitrios Watanabe, Naoki ![]() ![]() ![]() |
著者名の別形: | 山城, 佐和子 劉, 穎 渡邊, 直樹 |
キーワード: | Actin Focal adhesion Single-molecule biophysics |
発行日: | 20-Dec-2023 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 14 |
論文番号: | 8468 |
抄録: | Force transmission at integrin-based adhesions is important for cell migration and mechanosensing. Talin is an essential focal adhesion (FA) protein that links F-actin to integrins. F-actin constantly moves on FAs, yet how Talin simultaneously maintains the connection to F-actin and transmits forces to integrins remains unclear. Here we show a critical role of dynamic Talin unfolding in force transmission. Using single-molecule speckle microscopy, we found that the majority of Talin are bound only to either F-actin or the substrate, whereas 4.1% of Talin is linked to both structures via elastic transient clutch. By reconstituting Talin knockdown cells with Talin chimeric mutants, in which the Talin rod subdomains are replaced with the stretchable β-spectrin repeats, we show that the stretchable property is critical for force transmission. Simulations suggest that unfolding of the Talin rod subdomains increases in the linkage duration and work at FAs. This study elucidates a force transmission mechanism, in which stochastic molecular stretching bridges two cellular structures moving at different speeds. |
記述: | メカニカル・アンフォールディングが細胞内流動力を伝達する --走る電車の力を伝えるにはゴム人間が役に立つ--. 京都大学プレスリリース. 2024-01-12. |
著作権等: | © The Author(s) 2023 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/286625 |
DOI(出版社版): | 10.1038/s41467-023-44018-z |
PubMed ID: | 38123541 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2024-01-12-2 |
出現コレクション: | 学術雑誌掲載論文等 |
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