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smtd.202300999.pdf | 4.9 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Krishnamurthy, Kirankumar | en |
dc.contributor.author | Rajendran, Arivazhagan | en |
dc.contributor.author | Nakata, Eiji | en |
dc.contributor.author | Morii, Takashi | en |
dc.contributor.alternative | 中田, 栄司 | ja |
dc.contributor.alternative | 森井, 孝 | ja |
dc.date.accessioned | 2024-01-22T09:05:53Z | - |
dc.date.available | 2024-01-22T09:05:53Z | - |
dc.date.issued | 2024-01-19 | - |
dc.identifier.uri | http://hdl.handle.net/2433/286727 | - |
dc.description | DNA折り紙に革命を起こす --新たな応用を加速する新しい構造安定化法--. 京都大学プレスリリース. 2023-11-02. | ja |
dc.description.abstract | There have been limited efforts to ligate the staple nicks in DNA origami which is crucial for their stability against thermal and mechanical treatments, and chemical and biological environments. Here, two near quantitative ligation methods are demonstrated for the native backbone linkage at the nicks in origami: i) a cosolvent dimethyl sulfoxide (DMSO)-assisted enzymatic ligation and ii) enzyme-free chemical ligation by CNBr. Both methods achieved over 90% ligation in 2D origami, only CNBr-method resulted in ≈80% ligation in 3D origami, while the enzyme-alone yielded 31–55% (2D) or 22–36% (3D) ligation. Only CNBr-method worked efficiently for 3D origami. The CNBr-mediated reaction is completed within 5 min, while DMSO-method took overnight. Ligation by these methods improved the structural stability up to 30 °C, stability during the electrophoresis and subsequent extraction, and against nuclease and cell lysate. These methods are straightforward, non-tedious, and superior in terms of cost, reaction time, and efficiency. | en |
dc.language.iso | eng | - |
dc.publisher | Wiley | en |
dc.rights | © 2023 The Authors. Small Methods published by Wiley-VCH GmbH | en |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | cell lysate | en |
dc.subject | chemical ligation | en |
dc.subject | cosolvents | en |
dc.subject | DNA origami | en |
dc.subject | nuclease | en |
dc.title | Near Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Small Methods | en |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 1 | - |
dc.relation.doi | 10.1002/smtd.202300999 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 2300999 | - |
dc.address | Institute of Advanced Energy, Kyoto University | en |
dc.address | Institute of Advanced Energy, Kyoto University | en |
dc.address | Institute of Advanced Energy, Kyoto University | en |
dc.address | Institute of Advanced Energy, Kyoto University | en |
dc.identifier.pmid | 37736703 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2023-11-02 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 17H01213 | - |
datacite.awardNumber | 16K17934 | - |
datacite.awardNumber | 21K05274 | - |
datacite.awardNumber | 20H02860 | - |
datacite.awardNumber | 22H05418 | - |
datacite.awardNumber | 23H02083 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H01213/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K17934/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K05274/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H02860/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-22H05418/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H02083/ | - |
dc.identifier.pissn | 2366-9608 | - |
dc.identifier.eissn | 2366-9608 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 人工代謝経路を内包するナノ空間「複合触媒コンパートメント」の創出 | ja |
jpcoar.awardTitle | Development of new method for screening anticancer drugs that target topoisomerases by using DNA origami | en |
jpcoar.awardTitle | Retroviral integration into topologically-interlocked DNAs to probe the role of DNA structure and screen viral inhibitors | en |
jpcoar.awardTitle | DNAナノ構造体の階層的自己組織化による高効率な酵素連続反応場の構築 | ja |
jpcoar.awardTitle | DNAを構造ビルディングブロックとした酵素の集積状態の構築 | ja |
jpcoar.awardTitle | 代謝経路を内在する人工小器官の創製と機能発現原理の確立 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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