ダウンロード数: 23
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
dev.00652.pdf | 469.17 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Imai, Kaoru S. | en |
dc.contributor.author | Satoh, Nori | en |
dc.contributor.author | Satou, Yutaka | en |
dc.contributor.alternative | 今井, 薫 | ja |
dc.contributor.alternative | 佐藤, 矩行 | ja |
dc.contributor.alternative | 佐藤, ゆたか | ja |
dc.date.accessioned | 2024-05-20T04:10:24Z | - |
dc.date.available | 2024-05-20T04:10:24Z | - |
dc.date.issued | 2003-09 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287628 | - |
dc.description.abstract | Ascidian larvae develop mesenchyme cells in their trunk. A fibroblast growth factor (FGF9/16/20) is essential and sufficient for induction of the mesenchyme in Ciona savignyi. We have identified two basic helix-loop-helix (bHLH) genes named Twist-like1 and Twist-like2 as downstream factors of this FGF. These two genes are phylogenetically closely related to each other, and were expressed specifically in the mesenchymal cells after the 110-cell stage. Gene-knockdown experiments using a specific morpholino oligonucleotide demonstrated that Twist-like1 plays an essential role in determination of the mesenchyme and that Twist-like2 is a downstream factor of Twist-like1. In addition, both overexpression and misexpression of Twist-like1 converts non-mesenchymal cells to mesenchymal cells. We also demonstrate that the upstream regulatory mechanisms of Twist-like1 are different between B-line mesenchymal cells and the A-line mesenchymal cells called `trunk lateral cells'. FGF9/16/20 is required for the expression of Twist-like1 in B-line mesenchymal precursor cells, whereas FGF, FoxD and another novel bHLH factor called NoTrlc are required for Twist-like1 to be expressed in the A-line mesenchymal precursor cells. Therefore, two different but partially overlapping mechanisms are required for the expression of Twist-like1 in the mesenchymal precursors, which triggers the differentiation of the mesenchyme in Ciona embryos. | en |
dc.language.iso | eng | - |
dc.publisher | The Company of Biologists | en |
dc.rights | © 2003. Published by The Company of Biologists Ltd | en |
dc.rights | The full-text file will be made open to the public on 16 September 2004 in accordance with publisher's 'Terms and Conditions for Self-Archiving' | en |
dc.subject | Ascidian | en |
dc.subject | Ciona savignyi | en |
dc.subject | Ciona intestinalis | en |
dc.subject | Mesenchyme | en |
dc.subject | Basic helix-loop-helix Transcription factors | en |
dc.title | A Twist-like bHLH gene is a downstream factor of an endogenous FGF and determines mesenchymal fate in the ascidian embryos | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Development | en |
dc.identifier.volume | 130 | - |
dc.identifier.issue | 18 | - |
dc.identifier.spage | 4461 | - |
dc.identifier.epage | 4472 | - |
dc.relation.doi | 10.1242/dev.00652 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 12900461 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2004-09-16 | - |
datacite.awardNumber | 14704070 | - |
datacite.awardNumber | 13044001 | - |
datacite.awardNumber | 00J03588 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14704070/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13044001/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-00J03588/ | - |
dc.identifier.pissn | 0950-1991 | - |
dc.identifier.eissn | 1477-9129 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | ホヤ胚の内胚葉分化と中胚葉誘導における分子機構の解明 | ja |
jpcoar.awardTitle | 発生システムの構造と進化のメカニズム | ja |
jpcoar.awardTitle | ホヤ胚の内胚葉細胞分化決定機構の解析 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このリポジトリに保管されているアイテムはすべて著作権により保護されています。