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タイトル: | A Twist-like bHLH gene is a downstream factor of an endogenous FGF and determines mesenchymal fate in the ascidian embryos |
著者: | Imai, Kaoru S. Satoh, Nori Satou, Yutaka ![]() ![]() ![]() |
著者名の別形: | 今井, 薫 佐藤, 矩行 佐藤, ゆたか |
キーワード: | Ascidian Ciona savignyi Ciona intestinalis Mesenchyme Basic helix-loop-helix Transcription factors |
発行日: | Sep-2003 |
出版者: | The Company of Biologists |
誌名: | Development |
巻: | 130 |
号: | 18 |
開始ページ: | 4461 |
終了ページ: | 4472 |
抄録: | Ascidian larvae develop mesenchyme cells in their trunk. A fibroblast growth factor (FGF9/16/20) is essential and sufficient for induction of the mesenchyme in Ciona savignyi. We have identified two basic helix-loop-helix (bHLH) genes named Twist-like1 and Twist-like2 as downstream factors of this FGF. These two genes are phylogenetically closely related to each other, and were expressed specifically in the mesenchymal cells after the 110-cell stage. Gene-knockdown experiments using a specific morpholino oligonucleotide demonstrated that Twist-like1 plays an essential role in determination of the mesenchyme and that Twist-like2 is a downstream factor of Twist-like1. In addition, both overexpression and misexpression of Twist-like1 converts non-mesenchymal cells to mesenchymal cells. We also demonstrate that the upstream regulatory mechanisms of Twist-like1 are different between B-line mesenchymal cells and the A-line mesenchymal cells called `trunk lateral cells'. FGF9/16/20 is required for the expression of Twist-like1 in B-line mesenchymal precursor cells, whereas FGF, FoxD and another novel bHLH factor called NoTrlc are required for Twist-like1 to be expressed in the A-line mesenchymal precursor cells. Therefore, two different but partially overlapping mechanisms are required for the expression of Twist-like1 in the mesenchymal precursors, which triggers the differentiation of the mesenchyme in Ciona embryos. |
著作権等: | © 2003. Published by The Company of Biologists Ltd The full-text file will be made open to the public on 16 September 2004 in accordance with publisher's 'Terms and Conditions for Self-Archiving' |
URI: | http://hdl.handle.net/2433/287628 |
DOI(出版社版): | 10.1242/dev.00652 |
PubMed ID: | 12900461 |
出現コレクション: | 学術雑誌掲載論文等 |
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