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Title: <Original>Improvement on Preparation of RNA from Differentiating Xylem Tissue of Eucalyptus camaldulensis L.
Authors: BABA, Kei'ichi  kyouindb  KAKEN_id
ITO, Kazuya
Keywords: Eucalyptus
Issue Date: 30-Sep-1997
Publisher: Wood Research Institute, Kyoto University
Journal title: Wood research : bulletin of the Wood Research Institute Kyoto University
Volume: 84
Start page: 7
End page: 11
Abstract: The CTAB method for RNA isolation in differentiating xylem has improved in the treatment of tissues with methanol containing DTT and the addition of vanadyl ribonucleosides in to the extraction buffer. Vanadyl ribonucleosides were supposed to inhibit the binding of polyphenols to RNA also, not only inhibited RNase activity. The yield of RNA was more than 270 μg/g of tissue in average, and poly(A)^+ RNA was subsequently purified by using an oligo-dT cellulose column with about 0.7% recovery of total RNA.
Description: この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。
Appears in Collections:No.84

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