|Title:||<Original>Improvement on Preparation of RNA from Differentiating Xylem Tissue of Eucalyptus camaldulensis L.|
|Authors:||BABA, Kei'ichi |
|Publisher:||Wood Research Institute, Kyoto University|
|Journal title:||Wood research : bulletin of the Wood Research Institute Kyoto University|
|Abstract:||The CTAB method for RNA isolation in differentiating xylem has improved in the treatment of tissues with methanol containing DTT and the addition of vanadyl ribonucleosides in to the extraction buffer. Vanadyl ribonucleosides were supposed to inhibit the binding of polyphenols to RNA also, not only inhibited RNase activity. The yield of RNA was more than 270 μg/g of tissue in average, and poly(A)^+ RNA was subsequently purified by using an oligo-dT cellulose column with about 0.7% recovery of total RNA.|
|Appears in Collections:||No.84|
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